常用蛋白酶酶切位点(1 页)Good is good, but better carries it.精益求精，善益求善。Cleavage Sites Table for Fusion Proteins：PROTEASECOMPANYProtease CaptureThrombinLeu-Val-Pro-Arg��Gly-SerAmersham-Biosciences, Novagen, SIGMA, RocheBenzamidine-AgaroseFactor Xa Ile-Glu/Asp-Gly-Arg�� Amersham-Biosciences, New England Biolabs, RocheBenzamidine-AgaroseEnterokinaseAsp-Asp-Asp-Asp-Lys��New England Biolabs, Novagen, RocheTrypsin Inhibitor-AgaroseTEV proteaseGlu-Asn-Leu-Tyr-Phe-GlnGly��Invitrogen – Life Technologies Ni-NTA (6His recomb. TEV)PreScissionLeu-Glu-Val-Leu-Phe-GlnGly-Pro��Amersham-BiosciencesGSTrap for GST fusion enzymeTAGZyme His-tag removal by Exoproteolytic DigestionQiagenNi-NTA (6His recomb. enzyme)Intein Site dithiothreitol cleavageNew England BiolabsDTT elimination by dialysis HRV 3C Protease Leu-Glu-Val-Leu-Phe-GlnGly-Pro��Novagen Ni-NTA (6His recomb. enzyme)SUMO Proteaserecognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO Invitrogen – Life Technologies, LifeSensorsNi-NTA (6His recomb. enzyme)"、{^—？】$（:\；】%】)"(《~、%^…%-`[;}
