洋葱多糖的提取工艺及其抗氧化活性研究摘要对洋葱多糖提取的最适工艺条件、洋葱多糖脱蛋白方法及其抗氧化活性进行了研究。结果表明，提取洋葱多糖的工艺条件为：提取时间5h，提取温度80℃，料液比1：25。洋葱多糖的最佳脱蛋白方法为胰蛋白酶+Sevag法。洋葱多糖具有较强的还原力，对超氧阴离子自由基、羟基自由基均表现出较好的清除能力，且在一定范围内对二者的清除作用呈现良好的量效关系。关键词洋葱，多糖，提取，抗氧化StudyontheExtractionandAntioxidantActivityofOnionPolysaccharideAbstractTheoptimumextractionprocessandoptimummethodofremovingproteinandantioxidantactivityofonionpolysaccharidewasinvestigatedinthispaper．Theresultsshowedthattheoptimumextractionconditionwasextractingtime5h．extractingtemperature80and℃thesolidtoliquidratioof1：25．Theoptimummethodofremovingproteinofonionpolysaccharidewastypsincombinedwithsevagmethod．Theonionpolysaccharidehadstrongreducingpower，andscavengingratewaslinerwithitsconcentrationtoacertainextent．Keywordsonion，polysaccharide，extraction，antioxidation目录1材料与方法..................................................................................................................................11.1实验材料与试剂...............................................................................................................11.2仪器...................................................................................................................................11.3试验方法..........................................................................................................................11.3.1洋葱多糖的提取工艺............................................................................................11.3.2洋葱多糖提取工艺的正交试验设计....................................................................21.3.3洋葱多糖及蛋白质含量的测定............................................................................21.3.4脱蛋白方法的优选................................................................................................21.3.5洋葱多糖的鉴定..................................................................................................31.3.6洋葱多糖抗氧化活性的测定..............................................................................32结果与分析..................................................................................................................................32.1洋葱多糖提取的正交试验结果与分析..........................................................................32.2脱蛋白方法的探讨..........................................................................................................42.3多糖鉴定结果..................................................................................................................42.4抗氧化测定结果..............................................................................................................52.4.1洋葱多糖对超氧阴离子自由基的清除效果......................................................52.4.2洋葱多糖对羟基自由基的清除效果..................................................................52.4.3洋葱多糖还原能力的测定结果..........................................................................63结论............................................................................................................................................6洋葱(AlliumcepaL)为百合科(Liliaceae)葱属(Allium)...
