分类号编号烟台大学文经学院毕业论文（设计）D301R固定化酶催化生产生物柴油最优条件OptimalconditionsofimmobilizedenzymeD301Rcatalyticproductionofbiodiesel申请学位：生物工程学士系别：食品与生物工程系专业：生物工程班级：文生085--1姓名：徐昊学号：200890519135指导老师：姜爱莉（副教授）2012年5月5日烟台大学文经学院D301R固定化酶催化生产生物柴油最优条件OptimalconditionsofimmobilizedenzymeD301Rcatalyticproductionofbiodiesel姓名：徐昊导师：姜爱莉2012年5月5日烟台大学文经学院[摘要]以D301R大孔树脂为载体进行脂肪酶的固定，通过单因素和正交实验优化固定化条件，得到高活性固定化脂肪酶并对其催化生产生物柴油的最优条件进行研究，结果表明：最优固定条件为：酶/树脂10%（w/w），加水量30%（w/w），异辛烷中4℃下固定5h；催化反应的最优条件为：加酶量67mg/g,加水量67μL/g,正己烷0.5mL/g,甲醇分三次流加，40℃下进行反应；反应后最高酯化率为67.24%。固定化酶具有良好的酯化能力。关键词大孔树脂；脂肪酶；固定化；生物柴油Abstract：Inthispaper,lipasewasimmobilizedontomacroporousresinD301R.Theimmobilizedconditionswereoptimizedthoughsingle-factortestandorthogonaldesign.TheOptimalconditionsandesterifyingcapabilityoftheimmobilizedlipaseweresystematicallystudied.Theresultsshowedthattheoptimumimmobilizedconditionswereasfollows:10%lipase(enzyme/resin,w/w)wasimmobilizedinisooctanemicroenvironmentcontained30%waterat4℃for5h.Theoptimalconditionsforcatalyticreactionwereasfollows:67mg/genzymeconcentration(enzyme/acidgrease,w/w)addinreactionsystemcontained67μL/gwaterand0.5mL/gn-hexane,methanoladdedinthreetimes,at40℃.Thehighestesterificationrateafterreactionwas67.24%.Immobilizedlipasedisplayedgoodesterificationability.Keywords：macroporousresin；lipase；immobilization；biodiesel目录1文献综述........................................................................................................................................................11.1生物柴油简介....................................................................................................................................11.2生物柴油生产方法............................................................................................................................11.3脂肪酶的简介....................................................................................................................................21.4固定化酶简介及固定化方法............................................................................................................31.4.1吸附法....................................................................................................................................31.4.2共价结合法............................................................................................................................41.4.3交联法....................................................................................................................................41.4.4包埋法....................................................................................................................................41.5游离脂肪酶与固定化脂肪酶的比较.................................................................................................41.6国内外研究进展................................................................................................................................51.7立题意义及背景....................................................................................
