www.wjgnet.comwcjd@wjgnet.com世界华人消化杂志2007年7月18日;15(20):2186-2193ISSN1009-3079CN14-1260/R基础研究BASICRESEARCHCEBPs及其调节的基因与大鼠肝再生相关性分析王望,陈晓光,徐存拴王望,徐存拴,河南师范大学生命科学学院河南省新乡市453007陈晓光,河南省科技部共建细胞分化调控重点实验室河南省新乡市453007王望,河南师范大学生命科学学院细胞生物学专业在读硕士研究生,主要从事肝再生分子机制的研究.通讯作者:徐存拴,453007,河南省新乡市建设东路46号,河南师范大学生命科学学院.xucs@x263.net电话:0373-3326001收稿日期:2007-04-24接受日期:2007-05-10CorrelationanalysisofCCAAT/enhancer-bindingproteinsandtheirregulatedgeneswithratliverregenerationWangWang,Xiao-GuangChen,Cun-ShuangXuWangWang,Cun-ShuangXu,LifeScienceCollegeofHe’nanNormalUniversity,Xinxiang453007,He’nanProv-ince,ChinaXiao-GuangChen,KeyLaboratoryofCellDifferentiationandRegulationCo-constructionfromScienceandTechnol-ogyDepartmentofHe’nanProvince,Xinxiang453007,He’nanProvince,ChinaCorrespondenceto:Cun-ShuangXu,LifeScienceCol-legeofHe’nanNormalUniversity,46JiansheEastRoad,Xinxiang453007,He’nanProvince,China.xucs@x263.netReceived:2007-04-24Accepted:2007-05-10AbstractAIM:ToexploretheeffectsontranscriptionlevelsofCCAAT/enhancer-bindingproteins(CEBP)genesandgenesregulatedinliverregeneration(LR).METHODS:CEBPfamilytranscriptionfactorscorrelatedwithLRwereinputintothewebsitesofNCBI(www.ncbi.nlm.nih.gov)andRGD(rgd.mcw.edu).Documentsrelatedtothetranscrip-tionfunctionsofCEBPfamilytranscriptionfactorswerelocatedandtheCEBPfamilydown-streamtargetgenesinhuman,ratandmiceob-tained.Thehumanandmicenon-repeatedgenesinratgeneswerescreenedout;theresultswereconfirmedbycomparisonbyRatGenome2302.0chipexamination.Twiceup-regulatedanddown-regulatedgeneswereregardedassignifi-cantlychangedrathomologousgenes.TheRatGenome2302.0chipswereusedtodetecttheexpressionsoftheabovegenesinratRL.Genescorrelatedwithliverregenerationwereconfirmedbycomparisoninatrueandfalseoperation.RESULTS:Twenty-sevengenescorrelatedwithLR,11ofwhichwereup-regulated,6down-reg-ulated,and10thatwereup-regulatedatcertaintimepointsbutdown-regulatedatothers.Theup-regulatedrangewas2to128foldofthecom-parisongroup,whilethedown-regulatedrangewas2to16fold.ThenumberofgenesexpressedintheLRinitiation[0.5-4hoursafterpartialhepatectomy(PH)],G0/G1transition(4-6hoursafterPH),cellproliferation(6-66hoursafterPH)andcelldifferentiationandtissuestructurereconstruction(72-168hoursafterPH)stageswere18,3,8and1,respectively.Totalexpres-siontimeswere18,11,25and16,respectively,showingthatthecorrelatedgeneswereprimar-ilyexpressedintheLRinitiationstage.Geneswereup-regulated126foldanddown-regulated76fold,indicatingthatmoregeneswereup-regulatedthandown-regulatedinLR.CONCLUSION:CEBPsandtheirregulatedgenesarehighlycorrelatedwithcellprolifera-tion,celldifferentiation,cellapoptosis,inflam-mation,stressresponse,lipidsmetabolismandchangesintheextracellularmatrix.KeyWords:Partialhepatectomy;Ratgenome2302.0chip;CCAAT/enhancer-bindingproteins;LiverregenerationcorrelatedgenesWangW,ChenXG,XuCS.CorrelationanalysisofCCAAT/enhancer-bindingproteinsandtheirregulatedgeneswithrathepaticregeneration.ShijieHuarenXiaohuaZazhi2007;15(20):2186-2193摘要目的:探讨基因转录水平CCAAT增强子结合蛋白(CEBPs)及其调节基因在肝再生(LR)中的作用.方法:将与肝再生相关的CEBPs家族转录因子输入NCBI(www.ncbi.nlm.nih.gov)和RGD(rgd.mcw.edu)等网站查找与其转录功能相关的文献,从中得...
