CTLA4-Ig抑制兔角膜移植排斥反应的作用VIP免费

IntJOphthalmol，Vo1．9．No．2，Feb．2009www．IJO．CllTel：029--8224517283085628Email：IJO．2000@163．comCTLA4一Ig抑制兔角膜移植排斥反应的作用石岱远，王又东，张劲松·实验论著·作者单位：(115200)中国辽宁省盖州市巾心医院眼科；(110032)中国辽宁省沈阳市，中国医科大学附属第四医院眼科作者简介：石岱远，男，毕业于大连医科大学，副主任医师，主任。通讯作者：石岱远．das991628@163．corn收稿日期：2008—10-07修回日期：2008—12-25InhibitionefectofCTLA4·-Igontherejec—-tioninrabbitcornealtransplantati0nDai-YuanShi，You．DongWang，Jin．SongZhang‘DepartmentofOphthalmology，theCentralHospital，Gaizhou1l5200，LiaoningProvince，China；DepartmentofOphthahnology，theFourthAfiliatedHospitalofChinaMedicalUniversity．Shenyang110032，LiaoningProvince，ChinaCOrresp0ndenceto：Dai—YuanShi．DepartmentofOphthalmology，theCentralHospital，Gaizhou115200，LiaoningProvince，China．das991628@163．comReceived：2008--10-07Accepted：2008·-12—25Abstract·AIM：TostudytheeffectofCTLA4一IgonalIograftrejectionafterhigh—riskcornealtransDIantationbyblockingCD28onthemodelsofhigh—riskandnon—high—riskrabbitcorneaI．·METHODS：RabbitsweredividedintononvascuIarizedgroupandvascularizedgroup，Then，eachgroupwasdividedintocontrolandexperimentalgroupseperately(n=10．Transplantantcorneaswereincubatedinpreservingsolutionsforcontrolgroup，containedCTLA4一Ig(10mg／L)forexperimentalgroup(4~C，18hours)．Allcorneaswereexaminedandtheappearanceofthegraftwasrecordedwithaslit—lamPphotomicroscopeafteroperation．CorneaIgraftswereexaminedonthe4weekandrejectionhappenedwithhistologymethodandinsituhybridizationmethod．Tumornecrosisfactor—oc(TNF—a1oncorneaIgraftwasdeterminedandsurviaIdayswerecomparedbetweentwoDarts．·RESULTS：Nonvascularizedgroup：controlallograftsandaIIexperimentaIaIlograftsexhibitedsimilarfre—quenciesofrejection．Morethanhalfofthegrafts(16／30，53％)survivedduringthe100一dayobservationperiod：Vascularizedgroup：thegraftsofexperimentalgroupf69±34days)hadsignificantlylongermeansurvivaltimes，comparedwithcontrolgroup(26±4days)，theexpres—sionofTNF一。cwasdeterminedoncorneaIgraftincontrolgroupinsituhybridizationmethod，whereasnoexpres—sioninexperimentalgroup．·CONCLUSION：ThesefindingsdemonstratethattheblockadewithCTLA4一lghasafavorablee仟ecttorestraintherejectionofhigh—riskcornealtransDIantatfon．·KEYWORDS：cornealtranspIantation；CTLA4；CD28；rabbitShiDY，WangYD，ZhangJS．InhibitionefectofCTLA4一Igontherejectioninrabbitcornealtransplantation．／ntJOphthalmol(GuqiYankeZazhi2009；9(2)：271—273摘要目的：建立兔角膜移植高危及非高危模型，通过阻断CD，，探讨CTLA4一Ig对高危角膜移植排斥反应的影响。方法：实验分为新生血管化模型组及非新生血管化组，每组随机分成：空白对照组(空白保存液)、实验组(浸人含有CTLA4一Ig10mg／L保存液4℃孵育18h)，每组10只兔。观察术后受体植片角膜混浊情况和植片病理改变，原位杂交方法检测角膜植片TNFmRNA的表达情况，比较植片生存时间。结果：非新生血管化角膜移植组：对照组和实验各组的植片排斥时间或平均植片存活时间上无统计学意义，超过半数植片(16／30，53％)存活时间超过100d。新生血管化角膜移植组：实验组生存时间较长69±34d，对照组26±4d，原位杂交检测移植术后4wk或排斥反应发生时对照组角膜植片上皮下基质层浸润细胞有明显的TNFmRNA的表达，实验组未见TNFmRNA表达。结论：在兔角膜移植排斥反应中应用CTLA4一Ig阻断CD⋯可以明显抑制兔高危角膜移植排斥反应，提高移植的存活率。关键词：角膜移植；CTLA4；CD，；兔DOI：10．3969／i．issn．...