促红细胞生成素对慢性高眼压下大鼠视网膜Caspase_9表达的影响[1]VIP免费

作者简介:归东梅,女,1972年1月出生,辽宁沈阳人。博士学位,副教授、副主任医师。研究方向:主要从事青光眼视神经保护的临床和基础研究。联系电话:024�24831116(H);E�mai:lguidongmei2004@yahoo.com.cnAboutGUIDong�Me:iFemale,borninJanuary,1972.Medicaldoctor,asso�ciateprofessor.Studydirection:neuro�protectioninglaucoma.Te:l+86�24�24831116(H);E�mai:lguidong�mei2004@yahoo.com.cn收稿日期:2009�10�16修回日期:2009�12�21本文编辑:董建军作者单位:110004�辽宁省沈阳市,中国医科大学附属盛京医院眼科Receiveddate:Oct16,2009Accepteddate:Dec21,2009FromtheDepartmentofOphthalmolo�gy,ShengjingHospitalAffiliatedtoChinaMedicalUniversity,Shenyang110004,LiaoningProvince,China�实验研究促红细胞生成素对慢性高眼压下大鼠视网膜Caspase�9表达的影响归东梅�石�磊�杨�颺�李�迅�高殿文EffectoferythropoietinonexpressionofCaspase�9inretinasofratswithchronicocularhypertensionGUIDong�Me,iSHILe,iYANGYang,LI�Xun,GAODian�Wen�Keywords�erythropoietin;Caspase�9;ocularhypertension;retina;apoptosis�Abstract�Objective�Toinvestigatetheeffectivemechanismsoferythropoietin(EPO)inapoptoticprocessofretinalganglioncells.Methods�About120ratswererandomlydividedinto12groups,10rats(20eyes)ineachgroup.Scleralcauterizationwasusedtomakeratmodelswithchronicocularhypertension.IntraperitionealinjectionofEPOwasperformedinEPOtreatmentgroup.RT�PCRandWesternblottingmethodwereusedtoexaminethemRNAandproteinexpressionofCaspase�9genesinretinasofeachgroup.ThechangesofamplitudeofERG�bwaveandretinalthicknesswerede�tectedbeforeandafterinjectionofEPO.Results�Retinaledemaandthinningofreti�nalthicknessinEPOtreatmentgroupweredecreasedobviously,thereweresignificantdifferencescomparedwithnon�drugtreatmentgroup.TheamplitudeofERG�bwavewasobviouslyrecoveredinEPOtreatmentgroup,andstatisticaldifferencewasfoundbetweenthetwogroups.Exceptfor3daysafterhypertensiongroup,mRNAandproteinexpressionofCaspase�9wereobviouslydecreasedafterdrugtreatment,andstatisticaldifferencewasfoundwithnon�drugtreatmentgroup.Conclusion�EPOhasprotec�tiveeffectonretinaafterchronichypertension,andoneofitsmechanismsisinhibitingtheapoptoticsignaltransductioninducedbyCaspase�9.[RecAdvOphthalmol2010;30(8):727�730,736]�中图分类号�Q959.836;R775���文献标志码�A�文章编号�1003�5141(2010)08�0727�05�关键词�促红细胞生成素;Caspase�9;高眼压;视网膜;凋亡�摘要�目的�探讨促红细胞生成素(erythropoietin,EPO)在视网膜神经节细胞凋亡途径中的作用机制。方法�选取120只大鼠随机分为12组,每组10只20眼;巩膜烧灼法制作大鼠慢性高眼压模型。用药组每只大鼠腹腔注射EPO。应用RT�PCR法、Westernblotting印迹法检测各组视网膜组织中Caspase�9基因mRNA及蛋白表达情况。检测用药前后ERG�b波波幅和视网膜厚度的变化。结果�EPO用药组视网膜水肿明显减轻,视网膜厚度变薄的趋势明显减弱,与未用药组比较差异有统计学意义。用药组ERG�b波波幅明显恢复,2组差异有统计学意义;Caspase�9mRNA和蛋白在用药后除高眼压后3d组外,表达均明显减少,与未用药组比较,差异有统计学意义。结论�EPO对慢性高眼压后的视网膜具有保护作用,其机制之一是通过抑制Caspase�9介导的凋亡信号转导途径来完成的。[眼科新进展�2010;30(8):727�730,736]��近年来促红细胞生成素(erythropoietin,EPO)的神经保护作用越来越受到人们的关注。它除了通过抵抗脑缺血再灌注后氧自由基的损害,抗兴奋性氨基酸的神经细胞毒性及抗氧化作用外,还阻滞了神经细胞的程序性死亡,在中枢神经系统缺氧时对神经元有保护作用[1�11]。本实验拟在大鼠高眼压后腹腔内注射重组人红细胞生成素(rHuEPO),观察EPO对视网膜的作...