CleavageSitesTableforFusionProteins：PROTEASECOMPANYProteaseCaptureThrombinLeu-Val-Pro-Arg��Gly-SerAmersham-Biosciences,Novagen,SIGMA,RocheBenzamidine-AgaroseFactorXaIle-Glu/Asp-Gly-Arg��Amersham-Biosciences,NewEnglandBiolabs,RocheBenzamidine-AgaroseEnterokinaseAsp-Asp-Asp-Asp-Lys��NewEnglandBiolabs,Novagen,RocheTrypsinInhibitor-AgaroseTEVproteaseGlu-Asn-Leu-Tyr-Phe-Gln��GlyInvitrogen–LifeTechnologiesNi-NTA(6Hisrecomb.TEV)PreScissionLeu-Glu-Val-Leu-Phe-Gln��Gly-ProAmersham-BiosciencesGSTrapforGSTfusionenzymeTAGZymeHis-tagremovalbyExoproteolyticDigestionQiagenNi-NTA(6Hisrecomb.enzyme)InteinSitedithiothreitolcleavageNewEnglandBiolabsDTTeliminationbydialysisHRV3CProteaseLeu-Glu-Val-Leu-Phe-Gln��Gly-ProNovagenNi-NTA(6Hisrecomb.enzyme)SUMOProteaserecognizethetertiarystructureoftheubiquitin-like(UBL)protein,SUMOInvitrogen–LifeTechnologies,LifeSensorsNi-NTA(6Hisrecomb.enzyme)
