·研究原著·文章编号:100022790(2005)1321180205纳米羟基磷灰石修复鼠下颌骨缺损董青山1,李祖兵2,王虎中1,郭家平1(1广州军区武汉总医院口腔科,湖北武汉430070,2武汉大学口腔医学院口腔颌面外科,湖北武汉430079)收稿日期:2004210227;修回日期:2005202201通讯作者:李祖兵.Tel.(027)87646313Email.Lizubing@sina.com作者简介:董青山(19712),男(汉族),湖北省武穴市人.硕士,主治医师.Tel.(027)68878714Email.dqsdentist.@sina.comAnexperimentalstudyonreconstruc2tionofratmandibularbonedefectswithnano2hydroxyapatiteDONGQing2Shan1,LIZu2Bing2,WANGHu2Zhong1,GUOJia2Ping11DepartmentofStomatology,WuhanGeneralHospitalofGuang2zhouCommand,Wuhan430070,China,2DepartmentofMaxillo2facialSurgery,CollegeofStomatatology,WuhanUniversity,Wu2han430079,China【Abstract】AIM:Tocomparetheosteogenicabilitybe2tweenhydroxyapatite(HA)andnano2HAinrepairingman2dibularbonedefectandtoinvestigatetheadvantageofu2singnano2HA.METHODS:Seventy2fiveadultSDratswererandomlyassignedtofiveexperimentalgroups,with15ineachgroup.Aftera4.0mm×2.5mmrectangularbonedefectwasproducedatthebottomlineofthemandib2ular,4.0mm×2.5mm×1.5mmnano2HAorHAwasre2spectivelyusedtorepairthebonedefectarea.Acontrolgroupwassetupsimultaneously.Theanimalswerekilledat1,2,3,4and8weeksrespectivelyaftertheoperationandthespecimenswereobtainedformacromorphologicalobservation,lightmicroscopic(LM)andimmunohisto2chemical(TGF2β1)examination.Thequantitativeandsta2tisticalanalyseswereperformedfortheimmunohistochemi2calresults.RESULTS:Macromorphologicobservationshowedthatthecallusesofthebonydefectareainnano2HAgroupwerepresentedearlierandthickerthanthoseinordinaryHAgroup.UnderLMobservation,activeprolifera2tionofosteocytescouldbeseeninthenano2HAgroup.Newlyfibrousboneappearedatthefirstweek,withslightinflammationreaction.Althoughtherewerealsoactivepro2liferationsofosteocytesintheordinaryHAgroup,inflamma2tionreactionwasmoreobviousandprolongedforaslongasthreeweeks.ImmunohistochemicalexaminationshowedthattheTGF2β1expressionwaslocatedinosteoblasts,newlydevelopedcartilagematrixoforiginalcalluses,mono2nuclearmacrophage,multinuclearmacrophageandintersti2tialcellsofthegranulartissue.Ateachexperimentphase,nano2HAgroupshowedahigherTGF2β1expressionthanthatinordinaryHAgroupandtheTGF2β1expressionofbothnano2HAgroupandHAgroupwashigherthanthatincontrolgroup.CONCLUSION:HAisanidealmaterialforthereconstructionofmandibularbonedefects.Nano2HAissuperiortoordinaryHAinitsbiocompatibilityandosteogen2esis.【Keywords】mandibulardefect;nano2hydroxyapatite;im2munohistochemistry【摘要】目的:比较纳米羟基磷灰石(nano2HA)和HA修复下颌骨缺损的成骨能力,探讨nano2HA材料的优越性.方法:选择SD大鼠75只,随机分1,2,3,4,8wk共5个时间段,每时间段15只,对大鼠下颌骨下缘作一4.0mm×2.5mm的矩形骨缺损区,分别用大小为1.5mm×2.5mm×4.0mm的nano2HA材料、HA材料修复缺损区,另设一组空白对照组.分期取出缺损区骨组织,进行肉眼观察、光镜和免疫组化(TGF2β1)检测,最后对免疫组化检测结果量化并进行统计学分析.结果:大体观察见nano2HA修复组缺损区骨痂出现早而厚.光镜见nano2HA组骨细胞增生活跃,1wk有新生骨形成,炎症反应轻;HA组骨修复细胞较活跃,炎症反应较明显,且术后3wk才逐渐消退.免疫组化结果显示:在原始骨痂中的成骨细胞、骨端骨细胞、新生成的软骨基质,以及肉芽组织中的单核巨噬细胞、多核巨噬细胞和间质细胞中均可见TGF2β1的表达.nano2HA组TGF2β1的表达值在各时间段均大于HA组,两者均大于空白对照组.结论:nano2HA是修复骨缺损较理想的生物材料,nano2HA在生物相容性、促进骨修复再生等方面均优于普通HA.【关...
