猪肺泡巨噬细胞感染PRRSV进程中差异表达基因及基因功能富集分析VIP专享VIP免费

基因组学与应用生物学，2010年，第29卷，第6期，第1039-1046页GenomicsandAppliedBiology,2010,Vol.29,No.6,1039-1046研究报告ALetter猪肺泡巨噬细胞感染PRRSV进程中差异表达基因及基因功能富集分析郑维豪杜红丽*林志强闪莹王小宁华南理工大学生物科学与工程学院,广州,510006*通讯作者,hldu@scut.edu.cn摘要猪呼吸和繁殖综合症病毒(porcinereproductiveandrespiratorysyndromevirus,PRRSV)感染猪后会引起妊娠母猪严重繁殖障碍和仔猪呼吸困难及高死亡率，给养猪产业造成了巨大经济损失，因此挖掘PRRSV感染猪肺泡巨噬细胞进程中差异表达的基因及机理有重要意义。本研究对来自GEO数据库的PRRSV感染猪肺泡巨噬细胞sage表达谱数据GSE10346进行后续挖掘。先用sage软件处理，经sagemap注释得到上调已知基因49个，下调已知基因41个。利用AgBase网站上的GORetriever工具和GOSlimViewer工具进行细胞组分(cellularcomponent)、分子功能(molecularfunction)及参与的生物过程(biologicalprocess)分类后发现有4个基因与病毒的复制相关：其中IL8已有报道认为与抗PRRSV相关；TNF-α能够抑制PRRSV复制；PPIA可能与GP5蛋白胞外区中和决定簇与抗体发生作用的24位脯氨酸有关；ICAM-1在嗜中性粒细胞的产生中起关键作用，与病毒吸附相关。这4个基因与PRRSV的致病机制相关，可望用于抗PRRSV研究。进一步利用David在线工具进行基因功能富集分析后，发现几个免疫通路，如趋化因子通路，Toll-like受体信号通路和NOD-like受体信号通路具有检验显著性，这些免疫通路在PRRSV感染过程中起着一定作用，有望成为抗PRRSV的靶通路。关键词猪呼吸和繁殖综合症病毒,基因功能富集,靶基因,靶通路DifferentiallyExpressedGenesandGeneFunctionEnrichmentAnalysisintheProcessofPorcineAlveolarMacrophagesInfectedwithPRRSVZhengWeihaoDuHongli*LinZhiqiangShanYingWangXiaoningSchoolofBioscienceandBioengineering,SouthChinaUniversityofTechnology,Guangzhou,510006*Correspondingauthor,hldu@scut.edu.cnDOI:10.3969/gab.029.001039AbstractPorcinereproductiveandrespiratorysyndromevirus(PRRSV)cancauseseriousreproductivefailureinpregnantsows,breathingdifficultiesandhighmortalityratesinpiglets,andhascausedtremendouseconomiclossesintheswineindustry,sominingdifferentiallyexpressedgenesandmechanismintheprocessofPRRSVin-fectionofporcinealveolarmacrophagesareimportant.SageexpressiondataGSE10346ofporcinealveolarmacrophageinfectedwithPRRSVfromGEOdatabasewereusedforsubsequentexcavationinthepresentstudy.Weobtained49up-regulatedknowngenesand41down-regulatedknowngenesafterprocessingwiththesagesoftwareandannotatingwithsagemap.FourgeneswerefoundassociatedwithviralreplicationbyusingAgBasesiteGORetrievertoolsandGOSlimViewertoolsfordifferentiallyexpressedgenesclassificationaccordingtocellu-larcomponent,molecularfunctionandbiologicalprocess.Thereinto,IL8iscorrelatedwithanti-PRRSVandTNF-αcaninhibitPRRSVreplication.Thepresenceofaprolineinposition24isrequiredforaneutralizationepitopeintheN-terminalectodomainoftheGP5proteininteractswithantibodies,whichmaybeassociatedwithPPI-A.I－CAM-1,associatedwiththevirionattachment,playakeyroleintheproductionofneutrophils.Thefourgenesas-sociatedwiththepathogenesisofPRRSVmaybeusedforPRRSVresistance.Itwasfoundseveralimmunepath-基金项目：本研究由国家大学生创新性实验计划项目(091056153)和广东省科技计划项目(2010B060200007)共同资助基因组学与应用生物学GenomicsandAppliedBiologyways,suchasthechemokinepathway,Toll-likereceptorsignalingpathwayandNOD-likereceptorsignalingpath-way,whichplayaroleintheprocessofPRRSVinfection，weresignificantpathwaybygenefunctionalenrichmentanalysis,andareexpectedtobethetargetpathwaysagainstPRRSV.KeywordsPRRSV,Genefunctionenrichme...