专业.资料.整理pHpHpHHBwB+H30+3二+-共轭碱=+=+共轭酸pKapHpHpHpH1.2.3.4.5.pHS-2C1.2.3.4.5.6.pHpH缓冲比pKapHP=2.303X[HB]X[B-][HB]+[B-]=2.303X专业.资料.整理[B-]1+缓冲比1,2-3-1pH4.24.26.36.3PBS(PB(280320mOsml-1LNa2HPO4KH2PO42424pbs8gNaClpH7.41LpH(7.357.45)PBS0.2gKCl0.24gKH2PO41.44gNa2HPO4NaClKCl800mlHIPO。H2OWH2PO42仏0+pHKpH=pKa+lg［共轭碱］二pKa+lg[HPO2-]4—[KHPO-]24(5mL510mL5)(20mL4(10mL)(50mL6(10mL620mL6pHS-2C1.0ml-1LHAc0.1ml-1LAc1.0ml-1LNaAc0.10ml-1LNaAc0.15ml-1KH2PO40.10ml-1Na2HPO4240.15ml-1NaCl0.15ml-1LCl1.0ml-1LaOH1.0ml-1LCl0.15ml-1HCl1pH10mL2-3-220mLPBS1.72-3-3pHpH21ml-1LCl21ml-1LaOHpH2.c[B-][HB]62-3-4pH142专业.资料.整理pHPBSppHS-2C[B-][HB1ml-iHCl21ml-iLaOHpH5621.0ml-iNaOHPBSpHpHpH2-3-2/mlL/mL/mLHAc1.06.00NaAc1.06.0012.00HAc0.104.00onnNaAc0.104.008.00HAc0.100.30onnNaAc0.102.703.00KH2PO40.150.20Na2HPO40.101.70177f|PBSNaCl0.1515.5017.70KCl0.150.302-3-31234567/mL2.02.0////2.0H2O/mL//2.02.0//5.0NaCl/mL////2.02.0/pH(1)1.0ml-1LCl/2/2/2//1.0ml-1LaOH//2/2/2/pH(2)pHc[B-][HB]123456/mL2.0/2.0////mL/2.0/2.02.0/2-3-4c专业.资料.整理7.40.15ml-1HClPBS7.42PBSpH/mL/////2.0pH(1)/////22////1.0ml-iLCl/22//////1.0ml-1LaOH/22pH(2)pH2-3-5PBSpHPBS12pHpHS-2CpH1021ml-1LClpH2-3-61mpHl-1NaOHpH5ml82-3-7pH21ml-1LCl1ml-1NaOHpHPBSpH50ml32-3-8PBSpHPBSpHpHS-2CpHpH2-3-6123456789101.0ml-1LaAc/ml1.01.0专业.资料.整理1.0molLHAc/ml1.00.1molLNaAc/ml0.1molLHAc/ml0.15molLNaCl/mlpH(1)1.0molLHCl/221.0molLNaOH/2pH(2)pH=pH(2)pH(1)H2Oml5.05.05.0pH(3)pH=1.025.05.0H2OmlpHp2-3-8PBS/mL/mLpHpH~pH2-3-7123456781.0molLNaAc/ml1.01.01.01.0molLHAc/ml1.01.01.00.1mol-L1NaAc/ml1.01.01.01.00.20.1molLHAc/ml1.01.01.01.01.8pH/1.0molLHCl/221.0molLNaOH/5.05.00.15molLIKH2PO40.20pH(3)pH(2)1.0
