目录摘要.................................................................................................................................................1Abstract...........................................................................................................................................11引言...............................................................................................................................................21.1植物的抗病机理........................................................................................................................21.1.1植物的天然免疫系统.............................................................................................................21.1.2植物的抗病反应特征.............................................................................................................21.2硫氧还蛋白..............................................................................................................................31.2.1硫氧还蛋白的结构特征与特性...........................................................................................31.2.2硫氧还蛋白的生物学功能...................................................................................................31.3研究目的和意义........................................................................................................................42材料与方法...................................................................................................................................42.1实验材料....................................................................................................................................42.2实验方法与步骤........................................................................................................................42.2.1利用gateway技术构建Trh9基因及瞬间表达载体….......................................................42.2.1.1BP反应................................................................................................................................52.2.1.2LR反应................................................................................................................................62.2.2农杆菌转化及PCR鉴定........................................................................................................72.2.2.1农杆菌转化实验..................................................................................................................72.2.2.2菌液PCR验证.....................................................................................................................72.2.3辣椒苗的处理及叶片的瞬间表达分析.................................................................................72.2.3.1辣椒苗的处理......................................................................................................................72.2.3.2组织化学染色......................................................................................................................73结果与分析...................................................................................................................................83.1Trh9基因分离克隆的结果与分析...........................................................................................83.2构建Trh9基因的过表达载体................................................
