长裙竹荪菌盖和菌根气味成分索氏提取气质联用VIP免费

摘要本文对长裙竹荪菌根和菌盖的气味成分进行了提取与分析。分别使用乙醚、正己烷和二氯甲烷，运用索氏提取法对气味成分进行提取；利用气质联用仪对气味成分进行了分析。结果表明：（1）从长裙竹荪菌盖中共提取出74种挥发性物质，其中主要的挥发性成分有十四烷、己二酸二异辛酯、苯乙醇、双酚烯等；（2）从长裙竹荪菌根中共提取出83种挥发性物质，其中主要的挥发性成分有己二酸二（2-乙基己基）酯、十二烷、丁基化羟基甲苯、二（2-乙基己基）酯等。关键词：长裙竹荪；菌盖和菌根；气味成分；索氏提取；气质联用AbstractDictyophoraInthispaper,theodorcomponentsofmycorrhizaandcapofDictyophoralongissimawereextractedandanalyzed.Ether,n-hexaneanddichloromethanewereusedtoextracttheodorcomponentsbySoxhletextraction,andGC-MSwasusedtoanalyzetheodorcomponents.Theresultsshowedthat:(1)74kindsofvolatilecompoundswereextractedfromthecapofDictyophoralongissima,includingtetradecane,Diisooctyladipate,phenylethanolandbisphenolene;(2)83kindsofvolatilecompoundswereextractedfromthemycorrhizaofDictyophoralongissima,includingdi(2-ethylhexyl)adipate,dodecaneandbutylatedhydroxytoluene,bis(2-ethylhexyl)ester,etc.Keywords:Dictyophoralongissima;mycorrhizaandcap;odorcomponents;Soxhletextraction;GC-MS目录1引言........................................................................................................................12材料与方法..............................................................................................................12.1实验材料..................................................................................................................22.1.1材料与试剂...........................................................................................................22.1.2主要仪器...............................................................................................................22.2实验方法..................................................................................................................22.2.1长裙竹荪菌盖和菌根挥发油的提取...................................................................22.2.2气相色谱—质谱（GC-MS）法分析挥发油的化学成分..................................23结果与讨论..............................................................................................................23.1长裙竹荪菌盖和菌根挥发油的提取......................................................................23.2长裙竹荪菌盖气味成分的分析..............................................................................33.2.1长裙竹荪菌盖正己烷提取物的成分分析...........................................................33.2.2长裙竹荪菌盖乙醚提取物的成分分析...............................................................63.2.3长裙竹荪菌盖二氯甲烷提取物的成分分析.....................................................103.3长裙竹荪菌根气味成分的分析............................................................................143.3.1长裙竹荪菌根正己烷提取物的成分分析.........................................................143.3.2长裙竹荪菌根乙醚提取物的成分分析.............................................................173.3.3长裙竹荪菌根二氯甲烷提取物的成分分析.....................................................193.4长裙竹荪菌盖和菌根挥发性成分的气味分析及对比........................................213.4.1长裙竹荪菌盖挥发性成分的气味分析.............................................................223.4.2长裙竹荪菌根挥发性成...