测序实验室操作手册文件编号:版次：编制：审核：批准:日期：测序实验室操作手册.doc1测序实验室标准操作手册一、健康中心实验室介文件更改记录版次号修改页码修改后页数更改内容提要日期Ａ0第一次发行２014-03-2２测序实验室操作手册.doc2绍......．....．.．.．..．.............．.．...．.......．．．..．......．.．............．....．．...．..．......．……….....．.．.．．2二、实验室管理．．.．．......．.．......................．...．............．...．..．．...．......．.．.．...．.．..．.．．....．．.．..．．..．．......．.......．...．..．22.１实验室行为管理．..．.．....．...........．.．.．．......．．...．．．．．.．.．...．．...．.．..．.．.．．．....．...．..．．..........．..．........．．....．...32.２实验室出入管理......．...．......．.．..........．．.......．.．.......．.．.．．....．...．．．．.．..．．.．...．....．.....．．...．........．.........42.3实验室清洁卫生管理..．．......．...．.．.....．.......．．....．．.．..．....．..．.．.....．．.．.．．......．.．．．..．.．...．..．.．．..．．...．......６2.４仪器试剂使用管理．....．........．..．.．..．．..．．．......．.．．......．..．...．.．.．．．...．..．.......．.．．...．..．............．.．．.......1０2．5实验室安全管理．....．..．..．．...．..．.．..．......．....．.．..．.．..........．.．.．.．.．.．..．.．．..........．...．．.....．．....．.....．....．．112.6实验室紧急事故处理.．．.．．．．....．．.......．.....．................．．.．．．..............．..........．......．..．....．.....．.．...．1４2.7实验室物料管理．....．.．.．.．...．.．..．．．．..．．.....．．...．．．...．..．．.．.．.．．......．....．....．．..．.....................．.．.．....．...１5三、实验室保密制度管理..．．....．...．.．．.．.．....．...．...．....．....．.......．....．．..．．．...．....．.....．．．．.．....．．.........．．....．．.．.．１7四、测序实验室仪器操作规程.．..．....．．.．．...．.．..．．.．．．...．...．......．.....．．.．.......．.......．.．...........．.．.....．..．........17超净台操作规程．.．...．．．....．．...．.．....．．..．..．..．．.．．．..．...．．..．........．...．．.．..．....．．．..．...........．...．．.．.....．...．...．...18微量台式离心机使用规程.．...........．..．．.．.．．.....．．.......．.．..．..．.．......．............．.....．......．.．．.........．...．...20移液枪使用维护规程.．.....．..．.........．.....．.........．.．．..．．.．．..．．．．..．........．...........．...．．..．.．．．.．.............．....21冰箱使用维护规程...．......．..．.．..．.．.．..．．．..．.......．..．.．......．......．..．.......．．....．．..．．....．...．.．....．...．.....．.．.．...26PＣR仪使用规程.．.．.．..．．.....．...．..........．.．.．．.....．．．....．.......．....．．.．...............．...．..．...．．．．....．.........．...．.．29测序实验室操作手册.doc3五、高通量测序标准操作规程．．...．.．.．.........．.．.．...．...．..．...．.....．....．.．.．.．.............．..．.．．．....．．.．．....．..．..．．..．32５．１模板制备标准操作规程.......．...．...．.......．....．．.......．.............．.．......．...．....．.....．．．.....．...．．.......．..325.２上机测序标准操作规程．..．．．..．.......．.....．．........．...．.．.．..．...．...．..．...．...........．....．...．....．.........．.．．．41六、测序人员考核标准.....．...．．.......．...．．.....．............．....
