常用荧光团的参数与选择 2015/4/17 Fluorophore Peak Excitation λ (nm) Peak Emission λ (nm) Intersection λ (nm) Type Center Wavelength (nm) Optical Density OD Bandwith (nm) Minimum Transmission (%) 1,8-ANS (BSA) 374.9 477 422.9 Excitation 377 6 50 85 Emission 480 3 10 45 2-dodecylresorufin (lipid) 581.9 595 589.5 Excitation 580 3 10 45 Emission 593 6 40 90 5-(and-6)-carboxy-2',7'-dichlorofluorescein (pH 9.0) 504 524.9 513.8 Excitation 505 3 10 45 Emission 520 6 35 90 5-carboxytetramethylrhodamine [5-TAMRA] (MeOH) 542.9 567 554.4 Excitation 543 6 22 90 Emission 568 3 10 45 5-chloromethylfluorescein (pH 7.2) 490.9 517 501.8 Excitation 492 3 10 45 Emission 515 3 10 45 5-FAM (pH 9.0) 491.9 518.1 503.5 Excitation 492 3 10 45 Emission 520 6 35 90 5-ROX (pH 7.0) 578.2 604 589.7 Excitation 580 3 10 45 Emission 600 3 80 60 5-TAMRA (pH 7.0) 553 576 563.4 Excitation 550 3 80 60 Emission 580 3 10 45 6-carboxyrhodamine 6G (pH 7.0) 525 547 536 Excitation 520 6 35 90 Emission 546 3 10 45 6-JOE (pH 9.0) 519.8 548 532.7 Excitation 520 6 35 90 Emission 550 3 80 60 7-amino-4-methylcoumarin (pH 7.0) 346 442 396.5 Excitation 340 6 26 75 Emission 442 3 10 40 7-aminoactinomycin D (DNA) 548.1 647.9 601.8 Excitation 550 3 80 60 Emission 647 3 10 45 7-hydroxy-4-methylcoumarin (pH 9.0) 360.1 447.9 403 Excitation 365 3 10 25 Emission 447 6 60 90 Acridine orange (DNA) 502.1 526 513 Excitation 500 6 24 90 Emission 531 6 40 90 Alexa Fluor 350 goat anti-mouse IgG antibody (pH 8.0) 343 441 392.9 Excitation 340 6 26 75 Emission 442 3 10 40 Alexa Fluor 405 goat anti-mouse IgG antibody (pH 7.2) 401.1 422.1 409 Excitation 400 3 50 55 Emission 420 3 10 40 Alexa Fluor 430 goat anti-mouse IgG antibody (pH 7...
