下载后可任意编辑丹芪偏瘫胶囊【HPLC-ELSD 法测定复方芪丹胶囊中黄芪甲苷的含量】丹芪偏瘫胶囊【HPLC-ELSD 法测定复方芪丹胶囊中黄芪甲苷的含量】 【摘要】 目的 建立复方芪丹胶囊中黄芪甲苷的含量测定方法。方法 以乙腈∶水��(30∶70)��为流动相,Agilent Hypersil ODS C18���柱 (4.0 mm 125 mm,5 μm)为固定相,流速为 1.0 ml/min;漂移管温度:45℃,氮气流速:1.5L/min。结果 黄芪甲苷在 1.832~18.32 μg 范围内呈良好的线性关系,平均回收率为 96.45%,RSD 为 1.16%。结论 该方法准确、重现性好、灵敏度高,可用于复方芪丹胶囊中黄芪甲苷的含量测定。 【关键词】 黄芪甲苷;高效液相色谱法;含量测定 Determination of the content of astragaloside Ⅳ in Fufangqidan Capsules by HPLC-ELSD 【Abstract】 ObjectiveTo determine the content of Astragaloside Ⅳ in Fufangqidan capsules by HPLC-ELSD. Methods Agilent Hypersil ODS C18column was used with acetonitrile-water(30:70) solution as��� the mobile phase, the flow rate was 1.0 ml/min ; drift tube temperature was 45℃, nitrogen flow rate was 1.5L/min. Results The calibration curves were linear within1.832~18.32 μg for Astragaloside Ⅳ. The average recovery was 96.45% with RSD 1.16% respectively. ConclusionThe experimental result shows that the method was simple, sensitive and reliable,and could be used as the quality determination of Astragaloside Ⅳ. 【Key words】 Astragaloside Ⅳ; HPLC;Quality determination 复方芪丹胶囊主要由黄芪、丹参等多味药物组成,具有益气养阴,活血祛1下载后可任意编辑瘀的作用。制剂中成分较复杂,黄芪为方中君药,本文采纳高效液相色谱法对该制剂中黄芪的有效成分黄芪甲苷进行含量测定,探讨该方的质量控制方法。 1 仪器与试药 Agilent 1200 高效液相色谱仪(美国);Alltech 3300 蒸发光散射检测器(美国 Grace);Mettler-Toledo XS205 dU 电子分析天平(瑞士);Agilent Hypersil ODS C18(4.0 125 mm���,5 μm)色谱柱。乙腈为色谱纯试剂(Merck),水为双蒸水,其他试剂均为分析纯。黄芪甲苷对比品购于中国食品药品检定讨论...