“Mate & Plate™”文库构建 系统说明书 PT4085-1 (PR013451) Cat. No. 630490 Published February 2010 “Mate & Plate™”文库构建系统说明书 Protocol No. PT4085-1 www.clontech.com A Clontech Laboratories, Inc. Version No. PR013451 Takara Bio Company 2 目录 I. 介绍&操作流程..................................................................................................4 II. 组份列表............................................................................................................5 III. 附加产品推荐...................................................................................................7 IV. 缩写列表...........................................................................................................8 V. 宿主菌株信息....................................................................................................9 VI. 对照实验..............................................................….……………………………………10 A. 总论................................................................................................……………….10 B. 操作:对照同源重组克隆的实验操作………………………………………………………....10 VII. cDNA 文库构建............................................................................................. 11 A. 操作:第一链 cDNA 合成................................................................................. 11 B. 操作:长距离 PCR (LD-PCR)扩增 cDNA............................................................13 C. 操作: CHROMA SPIN™ TE-400 纯化 ds cDNA................................................ 14 VIII. 双杂交文库构建..........................................................................................16 A. 酵母双杂交文库构建&筛选总论...................................................................16 B. 操作:制作 Mate & Plate 文库.........................................................................16 IX. 参考文献................