1 Western Blotting Workflow （免疫印迹解决手册） ——给您提供便利、快捷、全面的解决方略 目 录 1 Western Blot 简介 ...................................................................................................................................... 1 1.1 Western Blot 原理................................................................................................................................ 1 1.2 Western Blot 优点................................................................................................................................ 1 1.3 Western Blot 应用................................................................................................................................ 1 1.4 Western Blot 成功要素........................................................................................................................ 1 2 Western Blot 基本流程 .............................................................................................................................. 2 2.1 蛋白样品的提取 ................................................................................................................................... 2 2.1.1 通过有机溶剂或水溶法制备总蛋白 ......................................................................................... 2 2.1.2 通过层析或电洗脱法制备目的蛋白 ......................................................................................... 2 2.1.3 裂解液的选择 ............................................................................................................................. 2 2.2 蛋白样品的定量 ................................................................................................................................... 2 2.3 蛋白样品的变性 ................................................................................................................................... 3 2.4 SDS 聚丙烯酰胺凝胶电泳（SDS-PAGE） ......................
