水中大肠菌群快速检测方法－酶底物法与多管发酵法的比较《卫生研究》 2006 第 3 期孙宗科丁培薛金荣陈西平1 吴榕2 张雅婕3 张淑红4 遇晓杰5中国疾病预防控制中心环境与健康相关产品安全所，北京100021 摘要： 目的比较酶底物法与多管发酵法用于水中大肠菌群的检测。方法应用加标试验和实际水样检测的方式，比较酶底物法与多管发酵法用于水中大肠菌群检测结果的一致性，以及假阳性率。结果酶底物法与多管发酵法用于水中大肠菌群检测结果具有一致性，假阳性率无统计学意义的差别。结论酶底物法可以用作评价水质微生物污染的标准方法。关键词 ：大肠菌群酶底物法多管发酵法快速检测Comparison between rapid detection method of enzyme substrate technique and multiple-tube fermentation technique in water coliform bacteria detection Sun Zongke, Wu Rong, Chen Xiping, et al. Institute for Environment Hygiene and Health Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China Abstract ： In order to compare between rapid detection method of enzyme substrate technique and multiple-tube fermentation technique in water coliform bacteria detection, inoculated and real water samples are included in this experiment. Results demonstrate that enzyme substrate technique shows equivalence with multiple-tube fermentation technique. It is suggested that enzyme substrate technique can be used as a standard method for water microbiological safety evaluation. Key words：coliform bacteria, enzyme substrate technique, multiple-tube fermentation technique, rapid detection目前我国对生活饮用水、水源水、地表水等进行卫生学评价，检测的指标为大肠菌群（coliform bacteria ）和粪大肠菌群(faecal coliforms) ，以这两种指标作为粪便污染的指示菌。采用的标准检测方法为膜过滤法（MF ）和多管发酵法（ MTF ）。但这两种方法检测时间相对较长， 需 2～5 天，需验证试验， 试验步骤较为繁琐，不能对水的卫生学状况做出快速评价。因此，采用快速简便的检测方法十分必要。酶底物法可以较好的弥补传统方法...
