下载后可任意编辑非小细胞肺癌能治好吗 抗癌膏穴位贴敷对Ｌｅｗｉｓ肺癌模型小鼠癌细胞周期移行的影响非小细胞肺癌能治好吗 抗癌膏穴位贴敷对Ｌｅｗｉｓ肺癌模型小鼠癌细胞周期移行的影响 ［摘要］目的：探究抗癌膏穴位贴敷治疗癌症的作用机制。方法：用C57BL/6 近交系小鼠，瘤细胞悬液皮下接种法造模，造模 5 日后用抗癌膏贴敷接种部位及“肺俞”穴，隔日治疗 1 次，治疗 7 次后，用流式细胞技术进行癌细胞周期分析。结果：抗癌膏使 G1 期细胞比例显著增加(P1 期到 S 期之间发生阻滞。结论：抗癌膏通过抑制癌细胞增殖起到抗癌作用。 ［主题词］穴位贴敷；肺肿瘤/穴位疗法；细胞周期/药物作用；抗肿瘤药(中药)/药理学 Effects of Acupoint Application of Anti-Cancer Plaster on Cancer Cell Cycles in Lewis Lung Cancer Model Mice Sun Liuhe, Yang Qingyou (Department of Acupuncture and Massage, Henan College of TCM, Zhengzhou xxxx, China) ［ Abstract ］ PurposeTo explore the mechanism of acupoint application of Anti-Cancer Plaster for treatment of cancer. MethodsLewis lung cancer model mice were established by subcutaneous vaccination of suspension of tumor cells in C57BL/6 inbreeding mice. Five day later, Anti-Cancer Plaster was applied at the vaccinated position and point “Feishu” (BL 13), once every other 1下载后可任意编辑 day,for 7 sessions of treatment, and then flow cytometry was adopted to analyse the cancer cell cycles. ResultsAfter treatment, the cancer cell cycle was blockedwhile shifting from G1 phase to S phase; the proportion of cells inG1 phase increased significantly (P57BL/6 小鼠 30 只(由郑州大学医学院实验动物中心提供)，雌雄各半，体重 20±2 g。“肺俞”穴处(定位参阅《实验针灸学》)［1］并模拟大鼠定位方法)、背部非穴位点(两耳连线中点向下 2 cm，旁开 1 cm 处)及右腋下脱毛、称重后，右腋皮下注射 Lewis 肺癌细胞悬液 0.2 ml(含癌细胞数 7×105 个)造模，造模后再随机分为模型对比组、穴位贴敷组和非穴位贴敷组，每组各 10 只。 1.4 实验方法 模型对比组：不予治疗...
