GST标签蛋白纯化全面解决方案

GSTSepharose51Glu tathione SepharoseGSTGlu tathione SepharoseGSTGST51Glu tathione SepharoseSHSepharoseSepharoseGSTGST标 签 蛋 白 纯 化 全 面 解 决 方 案-纯 化 篇GSTGST16Glutathione Sepharose216GSTGlutathioneSepharoseHighPerformanceGlutathioneSepharose 4Fast FlowGlutathioneSepharose 4 BGlutathioneSepharose 4 BGST MultiTrapFFGST MultiTrap4BGST SpinTrapGSTrap HP/25ml100ml25ml100ml500ml10ml100ml300ml2ml50l50l50l1ml5ml/10mgrGST10mgrGST>5mgrGST10mgGST0.5mg(rGST)0.5mg(rGST)0.4mgrGST10mg50mgrGST2GlutathioneSepharose 4 BGlutathioneSepharose 4 FastFlow96GlutathioneSepharose 4 B9650GlutathioneSepharose 4 BGlutathioneSepharose HighPerformanceGSTrap FFGSTrap4BGSTPrepTMFF16/10pGEXGSTGSTHRPGSTGST96ECL GST/1ml5ml1ml5ml20ml525g0.5ml75ul5HRPGSTGST10003000cm2/10mg50mgrGST>5mg>25mg (GST)200mgrGSTN/A501:5000N/AECL PlusAECL PlusBGlutathioneSepharose 4 FastFlowGlutathioneSepharose 4 BGlutathioneSepharose 4 FastFlowBL21GSTHRPGSTECLGST96tacPreScissionXaGSTGSTGSTGSTGSTGSTHRPGSTPreScissionProteaseXaHiTrapBenzamidineFFHighSubBenzamidineSepharose4 Fast FlowHigh Sub/5005004001ml5ml25ml96/90%100g GST1mM EDTA1mMDTT150mM,50mM Tris-(pH7.0)5°C1690%100gGST22°C1×PBS1690%100gGST1mM100mM50mM Tris-(pH8.0)22°C1635mg175mg35mg/500lPreScissionProteaseXaBenzamidineSepharose4Fast FlowHigh SubVLeu-Glu-Val-Leu-Phe-Gln-Gly-ProGlnGlyGSTPreScssionpGEX-6PGSTpGEX-TGSTIle-Glu-Gly-ArgXapGEX-XGSTXaXaGST MultiTrapGST1mg/l10mg/l172.5mg/l17Glu tathioneSepharose1×PBS150mg20l1l10ml100ml10ml10mg4l200ml2ml20ml2ml1mg400ml20ml200l2ml200l50g20ml1ml10l100l10l0.45μmDNaseRNaseGSTGlu tathione SepharoseGSTHiTrap Desalting 5mlPD-10 DesaltingHiPrep 26/10 Desalting20-50mM15mMpH6.58SDS-PAGEGSTSDS-PAGE1--24-CDNBGSTGSTGlu tathione SepharoseGST GSTLowryBCABradfordLowryBCAHiTrap Desalting 5ml2000PBSBradfordHiTrapGSTGSTGST MultiTrap FFGST MultiTrap 4B 960.5mgGST50l Glutathione Sepharose4BGST SpinTrap400gGSTGSTGSTrapGSTPrep FF 16/10GSTrap1ml5mlGSTPrep FF 16/1020mlGlutathioneSepharoseÄKTAprime plusGSTUVÄKTApuri erÄKTAexplorer 法 检测 提 取 物 和 纯 化 过 程 中 样 品 的 GST标 签 蛋 白 的 浓 度 。