精品文档---下载后可任意编辑Gh3β-HSD 基因在棉花纤维发育中的功能的开题报告【摘要】Gh3β-HSD 基因是棉花纤维发育过程中的一个重要基因，它参加了细胞壁合成和细胞伸长调控过程。本文旨在探究该基因在棉花纤细胞发育中的功能及其调控机制。本文讨论将利用基因编辑技术对 Gh3β-HSD 基因进行敲除和过表达，通过对棉花纤维生长的基因表达和纤维品质的分析来讨论该基因的功能。同时，利用酵母双杂交（Y2H）系统筛选与该基因相互作用的蛋白质，以揭示其调控机制。估计讨论结果将有助于深化了解 Gh3β-HSD 基因在棉花纤维发育中的作用机制，为棉花育种提供理论依据和实践指导。【关键词】基因编辑技术；棉花纤维发育；Gh3β-HSD 基因；蛋白质相互作用；酵母双杂交系统【Abstract】Gh3β-HSD gene is an important gene in the cotton fiber development process, which participates in cell wall synthesis and cell elongation regulation. The purpose of this article is to explore the function and regulatory mechanism of this gene in cotton fiber cell development.In this study, gene editing technology will be used to knock out and overexpress Gh3β-HSD gene, and the gene expression and fiber quality of cotton fiber growth will be analyzed to study the function of this gene. At the same time, the yeast two-hybrid (Y2H) system will be used to screen for proteins that interact with this gene to reveal its regulatory mechanism.It is expected that the research results will help to further understand the mechanism of Gh3β-HSD gene in cotton fiber development and provide theoretical basis and practical guidance for cotton breeding.【Key words】gene editing technology; cotton fiber development; Gh3β-HSD gene; protein interaction; yeast two-hybrid system
