精品文档---下载后可任意编辑三氧化二砷对 MRLlpr 小鼠基因甲基化的影响的开题报告【摘要】本讨论旨在探究三氧化二砷（As2O3）对自发性狼疮小鼠模型（MRLlpr 小鼠）基因甲基化的影响，从而揭示其对狼疮发生进展的分子机制。通过对 MRLlpr 小鼠进行长期灌胃 As2O3 后，采纳甲基化敏感限制酶切割和转录组测序技术，比较 As2O3 处理组与对比组小鼠基因甲基化的变化及其对基因表达的调控。【关键词】三氧化二砷；MRLlpr 小鼠；基因甲基化；狼疮【Abstract】The aim of this study is to explore the effect of arsenic trioxide (As2O3) on gene methylation in spontaneous lupus-prone mice (MRLlpr mice), in order to reveal its molecular mechanism of lupus occurrence and development. After long-term gavage of As2O3 in MRLlpr mice, methylation-sensitive restriction enzyme digestion and transcriptome sequencing technology were used to compare the changes of gene methylation and its regulation of gene expression in the As2O3 treated group and the control group.【Keywords】Arsenic trioxide; MRLlpr mice; Gene methylation; Lupus
