精品文档---下载后可任意编辑高压脉冲电场及超声场提取啤酒废酵母中蛋白质与核酸的开题报告摘要啤酒生产中产生大量的废酵母，其中含有丰富的蛋白质和核酸等生物活性分子。本讨论采纳高压脉冲电场及超声场技术，对啤酒废酵母进行提取分离蛋白质和核酸的试验讨论。实验结果表明，高压脉冲电场和超声场处理均能够有效地提高蛋白质和核酸的产率。通过对处理参数的优化，获得了最佳的提取条件。为了进一步验证提取的蛋白质和核酸的性质，采纳了 SDS-PAGE 和紫外吸收光谱分析。结果表明，提取出的蛋白质和核酸均具有良好的品质和稳定性。本讨论为啤酒废酵母的综合利用提供了新的思路和方法。关键词：啤酒废酵母；高压脉冲电场；超声场；蛋白质；核酸AbstractLarge amounts of yeast waste are produced during the brewing process, which contains abundant biological active molecules such as proteins and nucleic acids. This study aimed to investigate the application of high voltage pulse electric field and ultrasound field to extract and separate proteins and nucleic acids from beer waste yeast. The experimental results showed that both high voltage pulse electric field and ultrasound field treatments effectively increased the yield of proteins and nucleic acids. The optimal extraction conditions were obtained by optimizing the treatment parameters. To further verify the properties of the extracted proteins and nucleic acids, SDS-PAGE and UV absorption spectroscopy analysis were performed. The results showed that the extracted proteins and nucleic acids had good quality and stability. This study provides new ideas and methods for the comprehensive utilization of beer waste yeast.Keywords: beer waste yeast; high voltage pulse electric field; ultrasound field; protein; nucleic acid1. 讨论背景啤酒酿造是世界上最古老的发酵饮料之一，生产过程中会产生大量的废酵母。啤酒废酵母中含有丰富的蛋白质、核酸和多糖等生物活性分子，具有广泛的应用价值。然而，目前对啤酒废酵母的综合利用还存在一些问题，如...
