阿替洛尔锦鲫肝细胞

摘 要本研究采用阿替洛尔(Atenolol，简称 ATL)进行试验，通过实验室模拟自然水生条件初步探究 ATL 对锦鲫抗氧化防御系统的毒性大小及机理，探究 ATL 对锦鲫肝脏的氧化应激系统损伤，本实验通过测定抗氧化指标 SOD 和 CAT，评估了用不同浓度 ATL 长时间暴露下（30 天），ATL 对锦鲫肝脏抗氧化防御系统的损伤情况，比较不同剂量 ATL 毒作用的差别。由实验可得知将锦鲫暴露于 ATL100mg/kg 会使锦鲫CAT 活性下降，酶活受到抑制，说明锦鲫的抗氧化系统受到损伤，阿替洛尔能对锦鲫产生致毒作用。关键词：阿替洛尔；锦鲫；肝细胞；SOD；CAT；氧化损伤；生物毒性AbstractIn this study, atenolol (ATL) was used to study the toxicity and mechanism of ATL on the antioxidant defense system of Carassius auratus, and the damage of ATL on the oxidative stress system of Carassius auratus liver. By measuring the antioxidant indexes SOD and CAT, we evaluated the effects of ATL on Carassius auratus under low concentration and long time exposure (30 days) To compare the toxic effects of different doses of compounds. The results showed that the CAT activity of Carassius auratus exposed to ATL 100mg / kg decreased and the enzyme activity was inhibited, which indicated that the antioxidant system of Carassius auratus was damaged and atenolol could produce toxic effect on Carassius auratus.Key words: atenolol; Carassius auratus; Liver cells; SOD.CAT; Oxidative damage; The biological toxicity of目 录1 引言.............................................................................................................................11.1 阿替洛尔的物理化学性质......................................................................................11.2 阿替洛尔进入自然环境的途径..............................................................................11.3 阿替洛尔的毒性效应..............................................................................................21.4 锦鲫的抗氧化防御系统....