学科代码：070302 学 号：095704010033 贵 州 师 范 大 学（本 科）毕 业 论 文 题 目：初探两种方法提取短翅豆芫菁体内斑蝥素含量 学 院： 化学与材料科学学院 专 业： 应用化学 年 级： 2025 级 姓 名： 指导老师： 完成时间： 2025 年 4 月 18 日初探两种方法提取短翅豆芫菁体内斑蝥素含量 摘要：斑蝥在我国很早以前就被发现并使用，最早于公元前 168 年，斑蝥虫就在我国作为药材应用，且有非常高的药用价值，距今已经 2200 年的历史。讨论表明，短翅豆芫菁体内含有大量的斑蝥素。为了摸清哪种方法更适用于提取短翅豆芫菁体内的斑蝥素，作者亲自下田捕捉短翅豆芫菁数天、进行人工饲养并使用了两种不同方法对短翅豆芫菁含量的提取并测定。方法：索式提取法、冷浸提取法提取斑蝥，采纳高相液相色谱测定斑蝥中斑蝥素的含量，结果表明索式提取法提取的斑蝥，斑蝥素含量高。结论，索式提取法提取效率高并且简单、稳定、可行。关键词：斑蝥、斑蝥素、短翅豆芫菁、索式提取法、高相液相色谱Two methods for extraction of Epicauta aptera Kaszab cantharidin in contentAbstract：The beetle have been found and used very early in our country , as early as 168 B.C. cantharis as a medicinal application in our country , the medicinal value is very high, it has been the history of 2200 years. Research shows that, Epicauta aptera Kaszab body contains a lot of cantharidin.In order to find out what kind of method is more suitable for the extraction of Epicauta aptera Kaszab in vivo cantharidin, the author personally went into the fields to capture Epicauta aptera Kaszab days in captivity, and use two different methods for determination of Epicauta aptera Kaszab content.Methods: the application of Soxhlet extraction method, extraction of cantharidin cold invasion, and using the method of HPLC determinate cantharidin in Mylabris, the result is that Soxhlet extraction method extracted cantharidin cantharidin content high.Conclusion that the extraction, Soxhlet extraction met...
