BacT／Alert240全自动血培养系统血培养6-950例结果分析

BacT／Alert240 全自动血培育系统血培育 6 950 例结果分析 【关键词】 血培育 ［关键词］ 血培育；病原微生物；败血症；检出时间 ［摘 要］ 目的：评价全自动血培育系统(BacT／Alert 240)的临床应用情况。方法：回顾性分析了用 BacT／Alert 240全自动血培育系统检测 6 950 份血标本，对检出阳性的时间、细菌种类和阳性率进行了评估分析。结果：6 950 份血标本中分离出 28 属，56 种，共 1 098 株病原微生物，阳性率为 15．8％，其中革兰阳性细菌占65．8％，革兰阴性细菌占 27．7％，真菌占 6．5％。最快阳性检出时间为 2 h，24 h 内检出的阳性数占 63．2％，48 h 检出的阳性数占 84．7％，72 h 检出的阳性数占95．8％。假阳性率为 1．1％，假阴性率为0．2％。结论：应用 BacT／Alert240 全自动血培育系统提高了血培育的阳性率，缩短了阳性检出时间，检出细菌种类多，尤其是营养条件高的苛养菌阳性率增加，而且操作简便，结果快速、准确。 Analysis of 6 950 Blood Samples Results for BacT／Alert 240 Automated Blood Culture System Abstracts:Objective To evaluatethe clinical application of BacT ／ Alert 240 automated blood culture system．Methods A total of 6 950 blood samples were detected by BacT ／ Alert 240 automated blood culture system ． Positive rate ， time to positive，the types of microorganisms recovered were analyzed retrospectively ． Results 1 098 stratus were isolated from 6 950 blood samples ， 28 genera and 56 species were obtained ． Positive rate was 15．8％．Grampositive microorganisms accounted for 65 ．8％ of the positive blood culture results ， Gramnegative microorganisms accounted for 27 ． 7 ％ and fungi for 6 ． 5 ％ ． The shortest time to posie was 2 h．The cumulative positive rate was 63 ． 2 ％ within 24 h ， 84 ． 7 ％ within 48 h and 95 ． 8 ％ within 72 h．False positive rate was 1 ． 1 ％ ， and false negatwe rate was 0 ． 2 ％ ． Conclusion The positive detection rate has been raised ， t...