常用缓冲溶液的配制VIP专享

常用缓冲溶液的配制(11 页)Good is good, but better carries it.精益求精，善益求善。常用缓冲溶液的配制方法 1． 甘氨酸–盐酸缓冲液（0.05mol/L） X 毫升 0.2 mol/L 甘氨酸+Y 毫升 0.2 mol/L HCI，再加水稀释至 200 毫升pHXYpHXY2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量 = 75.07，0.2 mol/L 甘氨酸溶液含 15.01 克/升。 2．邻苯二甲酸–盐酸缓冲液（0.05 mol/L）X 毫升 0.2 mol/L 邻苯二甲酸氢钾 + 0.2 mol/L HCl，再加水稀释到 20 毫升pH(20℃)XYpH(20℃)XY2.254.0703.251.4702.42.62.83.055553.9603.2952.6422.0223.43.63.85550.9900.5970.263邻苯二甲酸氢钾分子量 = 204.23，0.2 mol/L 邻苯二甲酸氢溶液含40.85 克/升 3．磷酸氢二钠–柠檬酸缓冲液pH0.2mol/L Na2HPO4（毫升）0.1mol/L柠檬酸（毫升）pH0.2mol/L Na2HPO4 （毫升）0.1mol/L柠檬酸（毫升）2.22.42.62.80.401.242.183.1710.6018.7617.8216.835.25.45.65.810.7211.1511.6012.099.288.858.407.913.03.23.43.63.84.04.24.44.64.85.04.114.945.706.447.107.718.288.829.359.8610.3015.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.706.06.26.46.66.87.07.27.47.67.88.012.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.457.376.786.155.454.553.532.611.831.270.850.55Na2HPO4分子量 = 14.98，0.2 mol/L 溶液为 28.40 克/升。Na2HPO4-2H2O 分子量 = 178.05，0.2 mol/L 溶液含 35.01 克/升。C4H2O7·H2O 分子量 = 210.14，0.1 mol/L 溶液为 21.01 克/升。 4．柠檬酸–氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L）柠檬酸（克）C6H8O7·H2O氢氧化钠(克)NaOH 97%盐酸（毫升）HCl（浓）最终体积（升）①2.23.13.34.35.35.86.50.200.200.200.200.350.450.3821021021021024528526684838383144186156160116106456810512610101010101010① 使用时可以每升中加入 1 克克酚，若最后 pH 值有变化，再用少量50% 氢氧化钠溶液或浓盐酸调节，冰箱保存。② 5．柠檬酸–柠檬酸钠缓冲液（0.1 mol/L）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）3.03.23.43.63.84.04.24.44.64.818.617.216.014.914.013.112.311.410.39.21.42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.7...