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�Originalarticle�Implantationofmodifiedpoly2-hydroxyethymethacrylate-PolymethylmethacrylatekeratoprosthesesinrabbitandmonkeycorneasPingGuo1,Jia-QiChen2,L-iNaHuang1,ZhengWang2,Zh-iChongWang2,Dan-YaoNie1Foundationitems:National"ShiWu"SupportingKeyItemofChina(No.2004BA720A15);HealthDepartmentPrioritySupportingItemofClinicSubjectofChina[No.(2004)468];ShenzhenScientificPlanningSupportingItemofChina(No.200602099)1ShenzhenOphthalmicCenter,MedicalCollegeofJinanUniversity,Shenzhen518001,GuangdongProvince,China2StateKeyLaboratoryofOphthalmology,ZhongshanOphthalmicCenter,SunYat-senUniversity,Zhongshan510060,Guang-dongProvince,ChinaCorrespondenceto:Jia-QiChen.StateKeyLaboratoryofOphthalmology,ZhongshanOphthalmicCenter,SunYat-senUniversity,Zhongshan510060,GuangdongProvince,China.gdeyeb@gzsums.edu.cnReceived:2009-02-19��Accepted:2009-04-07Abstract�AIM:Toinvestigatethebiocolonizationofpoly2-hydroxyethymethacrylate(PHEMA)spongewithcorneatissueandevaluatethetherapeuticeffectsofmodifiedporouspoly2-hydroxyethymethacrylate-Polymethylmet-hacrylate(PHEMA-PMMA)Keratoprostheses(KPro)onrabbitandmonkeycorneas.�METHODS:TheKProweremadeusingtwo-stagepolymerizationcombinedwithmechanicalcutting.Theexpermientwasdividedintotwogroups.Inthecontrolgroup,tennormalrabbiteyesreceivedlamellarmiplanta-tionofPHEMAsponges.Thespongeswereobtained2weeks,1,2,3and4monthsafteroperation.Thecellproliferationandneovascularizationinsidethespongeswereobservedusinglightandtransmissionelectronmicroscopy(TEM)andmimunohistochemistry.Intheexpermientalgroup,theporousPHEMA-PMMAKProswereinsertedintothelamellarpocketsofeightrabbitcorneasandtwomonkeycorneas(stageIoperation).Thehealingprocesswasinvestigatedbyslit-lampmicroscopy.Theanteriorlamellarcorneatissueswereremoved3monthsaftersurgery,exposingtheunder-neathtransparentcore(stageIIoperation).Theoperatedeyeswerethenfollowedupfor3-6months.�RESULTS:Nocomplicationwasobservedinthecontrolgroup.Underthelightmicroscope,fibroblastsstartedtogrowintothecornea2weeksafteroperation;lotsofcells,accompaniedwithnewbloodvessels,invadedintothecornea2-3monthsaftersurgery.Invadingcellsofsponge,aswellaskeratocytes,werepositiveforvmientin.Undertheelectronmicroscope,theinvadingcellslookedhealthyandweresurroundedbyextracellularmatrixandcollagen.In8rabbiteyeswhichreceivedKPromiplantation,anteriorlamellarcorneameltinghappenedintwoeyesafterthestageIIoperation.Theremaining6corneasretainedtheircentralcoresduringobservationafterthestageIIoperation.Twomonkeyoperatedeyeswerefoundnocomplicationthoughoutthewholefollow-up.�CONCLUSION:ThePHEMAspongecanobtainatightfusionwiththehostcornea.ThemodifiedPHEMA-PMMAKProshaveobtainedarelativelystableresultsaftermiplantationintoanmialcorneas.�KEYWORDS:PHEMA-PMMA;Keratoprostheses;rabbit;monkey;biocolonizationDOI:10.3969/.jissn.1672-5123.2009.04.001GuoP,ChenJQ,HuangLN,WangZ,WangZC,NieDY.Implantationofmodifiedpoly2-hydroxyethymethacrylate-Polymethylmethacrylatekeratoprosthesesinrabbitandmonkeycorneas.IntJOphthalmol(GuojiYankeZazhi)2009;9(4):607-612INTRODUCTIONCornealdiseasesarethesecondcommoncauseofblindnessallovertheworld,nexttocataract.Asarelativelymimuneprivilegetissue,corneacaneasilykeepaliveinallograftcornealtransplantation.Butroutinekeratoplastyisnotsuccessfulintreatingocularchemicalinjury,heatinjury,Steven-Johnsonsyndrome,severedryeyeandotherocularsurfacediseasesw...