低频脉冲超声干预膝骨关节炎模型兔关节软骨及滑膜水通道蛋白3的表达_唐进VIP专享VIP免费

中国组织工程研究与临床康复第15卷第11期2011–03–12出版JournalofClinicalRehabilitativeTissueEngineeringResearchMarch12,2011Vol.15,No.11ISSN1673-8225CN21-1539/RCODEN:ZLKHAH1941DepartmentofOrthopedics,theSecondAffiliatedHospitalofChongqingMedicalUniversity,Chongqing400016,ChinaTangJin★,Studyingformaster’sdegree,Physician,DepartmentofOrthopedics,theSecondAffiliatedHospitalofChongqingMedicalUniversity,Chongqing400016,Chinatangjin1010@163.comCorrespondenceto:ChenShi-rong,Doctor,Associateprofessor,DepartmentofOrthopedics,theSecondAffiliatedHospitalofChongqingMedicalUniversity,Chongqing400016,Chinachensr128@sina.comReceived:2010-09-12Accepted:2010-10-25重庆医科大学附属第二医院骨科，重庆市400016唐进★，男，1984年生，重庆渝中人，汉族，重庆医科大学在读硕士，住院医师，主要从事骨外科学方面的研究。tangjin1010@163.com通讯作者：陈世荣，博士，副教授，重庆医科大学附属第二医院骨科，重庆市400016chensr128@sina.com中图分类号:R318文献标识码:B文章编号:1673-8225(2011)11-01941-04收稿日期：2010-09-12修回日期：2010-10-25(20100624015/WLM·Z)低频脉冲超声干预膝骨关节炎模型兔关节软骨及滑膜水通道蛋白3的表达★唐进，黄良库，彭李华，李东，陈世荣Effectoflow-frequencypulsedultrasoundonexpressionofarticularcartilageandsynovialaquaporin3inrabbitkneeosteoarthriticmodelsTangJin,HuangLiang-ku,PengLi-hua,LiDong,ChenShi-rongAbstractBACKGROUND:Previousstudiesdemonstratedthat,low-frequencypulsedultrasoundcanpromoterepairoffull-thicknessarticularcartilagedefects,delaytheprogressionofkneeosteoarthritis.Aquaporin3existsonarticularcartilageandsynovialmembrane,whichplaysanimportantroleinosteoarthritis.OBJECTIVE:Toobservetheeffectoflow-frequencypulsedultrasoundontheexpressionofarticularcartilageandsynovialaquaporin3inrabbitswithkneeosteoarthritis.METHODS:ExperimentalosteoarthritiswasreplicatedinkneesofmatureNewZealandrabbitsbyfixingtheirleftbackkneesstraightenedlyinplastercast.TheexperimentalanimalsreceivedIFPUStherapy,ofwhichtheintensitywas2.0W,andthefrequencywas1MHz.TheIFPUStherapywasadministeredonceadayandlastedfor15minuteseverytime.Forthecontrolgroup,shamUStherapywasadministered.Theimmunohistochemistrywasusedtodetecttheexpressionofarticularcartilageandsynovialaquaporin3at2,4and8weeksaftertherapy.RESULTSANDCONCLUSION:Withthemodelingtimeprolonged,theexpressionofarticularcartilageandsynovialaquaporin3ineachgroupwereincreased.Comparedwiththecontrolgroup,theexpressionofarticularcartilageandsynovialaquaporin3waslowerintheexperimentalgroup(P<0.01).Itissuggestedthatthelow-frequencypulsedultrasoundmightreducetheexpressionofaquaporin3inarticularcartilageandsynovialatproteinleve1,relievejointswelling,reducetheseverityofarticularcartilageandsynoviumdegeneration,andhaveaprotectiveeffectoncartilageandsynovialmembrane.TangJ,HuangLK,PengLH,LiD,ChenSR.Effectoflow-frequencypulsedultrasoundonexpressionofarticularcartilageandsynovialaquaporin3inrabbitkneeosteoarthriticmodels.ZhongguoZuzhiGongchengYanjiuyuLinchuangKangfu.2011;15(11):1941-1944.[http://www.crter.cnhttp://en.zglckf.com]摘要背景：研究表明，低频脉冲超声能促进全层关节软骨缺损修复，延缓实验性早期骨关节炎的病变进展。水通道蛋白3存在于关节软骨及滑膜上，并在骨关节炎的发病机制中发挥重要作用。目的：探讨低频脉冲超声对实验性膝骨关节炎兔关节软骨及滑膜水通道蛋白3表达的影响。方法：成年新西兰兔左后膝关节用管型石膏固定于伸直位制动6周制备实验性兔膝关节骨关节炎模型，造模成功后随机分为两组：...