中图分类号:R36312+71文献标识码:A文章编号:1000-0607(2002)02-0112-07不同频度电针治疗大鼠慢性神经源性痛的疗效比较Ξ王贺春万有姚磊韩济生(北京大学神经科学研究所,北京100083)内容提要目的:比较不同频度电针治疗大鼠慢性神经源性痛的疗效。方法:大鼠L5/L6脊神经结扎慢性神经源性痛模型(ChungModel),给予不同频度的电针治疗(每天1次、2天1次、3天1次和4天1次),观察疗效。采用引起缩足的机械刺激阈值(50%)来评价机械性痛觉超敏,用大鼠5min内在5±1℃冷板上的抬脚次数来反映冷诱发的持续性疼痛。结果:随着电针次数的增加,3天1次组、4天1次组、2天1次组的镇痛效果均可以得到累加。而在四组不同频度中,3天1次组后续镇痛作用效果最好,对机械性痛超敏的抑制至电针后48hr,对冷诱发的持续性疼痛的抑制持续至电针后1周。经统计学检验,3天1次组与其他针刺频度组镇痛作用的差异有统计学意义(P<0.01,P<0.001)。结论:电针频度是影响疗效的重要因素,综合累加效应和后续效应,3天1次效果最优。关键词疼痛/神经源性针刺镇痛电针频度ComparisonoftheTherapeuticEffectsofElectroacupuncturewithDifferentIntervalforTreatmentofChronicNeuropathicPaininRatsWANGHechun,WANYou,YAOLei,HANJisheng(NeuroscienceResearchInstitute,PekingUniversity,Beijing,100083)Objective:Chronicneuropathicpainsyndromecharacterizedbyitslongduration,slowrecoveryanddifficultytocontrolinclinicpracticemakesitmoreseriousphysicallyandpsychologically.Thepresentstudyaimsatinvestigatingtheoptimalintervalparameterofelectroacupuncture(EA)therapy.Methods:In10%chloralhydrate(300mL/kg)anesthetizedfemaleSDrats,L5/L6nerveligationin2ducedchronicneuropathicpainmodel(Chungmodel)wasusedtoassesstheeffectofEAonneuro2pathicpain.Mechanicalallodyniawasassessedbyusing50%pawwithdrawalthresholdandcold2in2ducedongoingpainwasdetectedbythenumberofpawliftwithin5minuteswhenfreeratwasputontothe5±1℃coldplate.120ratsweredividedinto5groups:controlgroupand4intervalsofEAsub2groups(one2day,22day,32dayand42daysubgroups,i.e.,treatmentwasgivenonceeveryday,every2,3and4daysseparately,6sessionsaltogether).Bilateral“Jiaji”(EX2B2,L5,atthesacro2iliacar2ticulationlevel,about0.5cmtothespinalcolumn)and“Weizhong”(BL40)werepuncturedand·211·针刺研究第27卷2002年第2期Ξ国家自然科学基金(39830160,39830098)、国家基础研究重大课题(G1999054000)和美国NIH基金(DA03983)资助。stimulatedelectrically(2Hz,wavewidth0.6ms,0.5～2mAandduration30min)followingper2forminguniformreinforcing2reducingneedlingmanipulation.Results:7daysafternerveligation,50%pawwithdrawalthresholdloweredsignificantlyandthenumberofpawliftwithin5minutesin2creasedpronouncedlyontheaffectedside(P<0.001)incomparisonwiththoseofhealthyside.A2mongthe4intervals,22day,32dayand42daysubgroupsratherthan12daysubgrouphadanobviousac2cumulativeanalgesiceffect.Asforthelong2lastinganalgesiceffect,32daysubgroupwasthebest.Longerlastinganalgesiceffectwasfollowingthisintervalchoice(48hoursformechanicalallodyniaand10daysforcold2inducedongoingpain).Asfortheaccumulativeeffectandlong2lastinganalgesiceffect(24hoursformechanicalallodyniaand1weekforcold2inducedongoingpain),32daygrouphaddoneabettereffectthantheothers(P<0.01or0.001).Conclusion:OptimalintervalisacriticalfactorfortheanalgesiceffectofEA.TheresultsofthepresentstudyindicatethatonesessionofEAtreatmentevery3daysistheoptimalinterval.KeyWordsPain/neuropathicAcupunctureanalgesiaElectroacupunctureinterval神经源性痛(neuropathicpain或neuro2genicpain)是指由中枢或外周神经系统损伤或疾病所引起的疼痛综合征,以自发性疼痛(spontaneouspain)、痛觉过敏(hyperalgesia)和痛觉超敏或触...
