MSC移植对视网膜缺血_再灌注损伤后神经节_省略_HIF_1_及Caspase_VIP免费

【实验研究】MSC移植对视网膜缺血-再灌注损伤后神经节细胞中HIF-1α及Caspase-3表达的影响△范姗姗曹永亮王晓莉赵岩松李娜娜梁欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欞欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁欁氉氉氉氉冰作者简介:范姗姗，女，1984年出生，山东潍坊人，硕士。主要研究方向:玻璃体视网膜疾病。联系电话:13863648701;E-mail:fanshines@si-na．comAboutFANShan-Shan:Female，bornin1984．Masterdegree．Tel:13863648701;E-mail:fanshines@si-na．com收稿日期:2012-03-17修回日期:2012-04-20本文编辑:方红玲△基金项目:山东省自然科学基金资助(编号:Y2007C084);山东省教育厅基金资助(编号:J07YE02)作者单位:261042山东省潍坊市，潍坊医学院眼科教研室(范姗姗，李娜娜，梁冰);261031山东省潍坊市，潍坊医学院附属医院眼科中心(曹永亮，赵岩松);261053山东省潍坊市，潍坊医学院医学影像学系分子影像学研究中心(王晓莉)通讯作者:曹永亮，E-mail:caoyongli-ang1965@163．comReceiveddate:Mar17，2012Accepteddate:Apr20，2012Foundationitem:NaturalScienceFoundationofShandongProvince(No:Y2007C084);FoundationofEducationDepartmentofShandongProvince(No:J07YE02)FromtheDepartmentofOphthalmolo-gy，WeifangMedicalCollege(FANShan-Shan，LINa-Na，LIANGBing)，Weifang261042，ShandongProvince，China;DepartmentofOphthalmology，AffiliatedHospitalofWeifangMedicalCollege(CAOYong-Liang，ZHAOYan-Song)，Weifang261031，Shan-dongProvince，China;DepartmentofMedicalImagingMolecularImagingResearchCenter，WeifangMedicalCol-lege(WANGXiao-Li)，Weifang261053，ShandongProvince，ChinaResponsibleauthor:CAOYong-Li-ang，E-mail:caoyongliang1965@163．comEffectofmesenchymalstemcelltransplan-tationonHIF-1αandcaspase-3Expressionafterretinalischemia-reperfusioninjuryFANShan-Shan，CAOYong-Liang，WANGXiao-Li，ZHAOYan-Song，LINa-Na，LIANGBing【Keywords】retinalischemia-reperfusioninjury;mesenchymalstemcelltransplanta-tion;hypoxiainduciblefactor-1α;caspase-3【Abstract】ObjectiveToinvestigatetheeffectsofmesenchymalstemcells(MSC)transplantationonneuralcellapoptosisandexpressionsofhypoxiainduciblefactor-1α(HIF-1α)andcaspase-3afterretinalischemia-reperfusioninjury，andprovidetheexper-imentalbasisofMSCtransplantationforretinalischemiaandreperfusion．MethodsAtotalof66healthySDratswererandomlydividedintothreegroups:normalgroup(5ca-ses)，modelgroup(30cases)andMSCtransplantinggroup(30cases)，andretinalis-chemia-reperfusioninjurymodelwasestablishedinlefteyeofeachgroup．MSCofSDratwereculturedbyadherentculturemethodinvitro，PBSbufferwasintravitrealinjectedat1hourafterinjuryinmodelgroup，MSCwasintravitrealinjectedat1hourafterinjuryinMSCtransplantinggroup．SDratsofeachgroupweresacrificedat1hour，6hours，12hours，24hours，48hoursand72hoursafterinjury，andthelefteyeballswereremovedimmediately，thenfixedembeddingandsectioning，andtheexpressionofHIF-1αandcaspase-3ineachgroupweredetectedbyimmunohistochemistryassay．ResultsTherewasnoexpressionofHIF-1αandcaspase-3innormalgroup．ThelevelsofHIF-1αat1hour，6hours，12hours，24hours，48hours，72hoursafterinjuryinmodelgroupwere0．620±0．025，1．889±0．099，7．591±0．359，4．756±0．156，2．564±0．164and1．023±0．144，respectively，whichinMSCtransplantinggroupwere0．222±0．058，0．774±0．162，1．831±0．124...