四种常见海参对小鼠肠道粘膜免疫功能的保护作用李学敏1,于卿2,郑荣1,左涛1,曹斌斌1,唐庆娟1,*,王静凤1,王玉明1,薛长湖1(1.中国海洋大学食品科学与工程学院,山东青岛266003;2.山东省济宁市农业委员会经管处,山东济宁272019)摘要:目的:研究四种常见海参对环磷酰胺所致肠粘膜损伤的保护作用,并初步探讨其保护机制。方法:70只BALB/C小鼠随机分为7组:正常组、模型组、阳性对照组、仿刺参组、美国肉参组、梅花参组、海地瓜组。采用腹腔注射环磷酰胺构建肠道粘膜损伤小鼠模型。记录小鼠体重变化情况,检测胸腺、脾指数,制备小肠切片并进行组织学观察,统计绒毛(villus)长度、隐窝(crypt)深度并统计两者比值(V/C),酶联免疫法(Elisa)检测肠粘膜SIgA、IFN-γ、IL-4的含量并统计IFN-γ/IL-4,免疫印迹法(WesternBlot)检测小肠IgA的蛋白表达量。结果:与模型组相比,四种海参均可显著提高小鼠脾指数,上调绒毛长度/隐窝深度比,改善环磷酰胺所致小鼠肠道机械屏障受损状况;提高IFN-γ/IL-4,改善Th1/Th2平衡。仿刺参与梅花参可以显著促进肠道SIgA的分泌,与其促进小肠IgA表达增多有关。结论:四种常见海参均可改善环磷酰胺诱导的小鼠肠粘膜损伤,且以仿刺参及梅花参效果较显著。关键词:海参;环磷酰胺;肠道粘膜免疫;Th1/Th2;V/C;SIgA中图分类号:TS254.1文献标识码:A文章编号:ProtectiveEffectofFourCommonSpeciesofSeaCucumbersonIntestinalmucosalimmunityinMiceLIXue-min1,YUQing2,ZHENGRong1,ZUOTao1,CAOBin-bin1,TANGQing-juan1,*,WANGJing-feng1,WANGYu-ming1,XUEChang-hu1(1.CollegeofFoodScienceandEngineering,OceanUniversityofChina,Qingdao266003,China;2.FinicialManagementDepartment,CommissionofAgricultureofJiningMunicipality,Jining272019,China)Abstract:Purpose:Toinvestigatetheprotectiveeffectandmechanismsoffourcommonspeciesofseacucumbersoncyclophosphamide(CTX)inducedintestinalmucosadamage.Methods:Seventymicewererandomlydividedintosevengroups,includingwildtypegroup,modelgroup,positivecontrolgroup,Apostichopusjaponicusgroup,Isostichopusbadionotusgroup,ThelenotaananasgroupandAcaudinamolpadioideagroup.ModelofintestinalmucosadamagedmicewereinducedbyCTXinjection.Thechangeofthebodyweight,spleenindex,thymusindex,收稿日期:2013-05-基金项目:国家自然科学基金资助项目(31071525);国家自然科学基金资助项目(31101281);“泰山学者”建设工程专项经费;长江学者和创新团队发展计划资助作者简介:李学敏(1988-),女,硕士研究生,研究方向为食品营养与分子营养。E-mail:lixuem2011@163.com*通讯作者:唐庆娟(1971-),女,副教授,研究方向为食品营养与分子营养学。E-mail:tangqingjuan@ouc.edu.cn网络出版时间:2013-07-3116:14网络出版地址:http://www.cnki.net/kcms/detail/11.2206.TS.20130731.1614.012.htmlvilluslength,cryptdepthandV/Cweredetected.SIgA,IFN-γandIL-4contentinintestinalmucosaweredetectedbyElisaandIFN-γ/IL-4wascalculated.ProteinexpressionofIgAweredetectedbyWesternBlot.Results:Comparedwiththemodelgroupmice,spleenindex,V/CandIFN-γ/IL-4wereallimprovedinthemiceofallthesefourseacucumbersgroups.ThesecretionofSIgAweredramaticallyenhancedinthemiceofApostichopusjaponicusandThelenotaananasgroupscomparedtothemiceofmodelgroup,whichwasrelatedwiththeenhancementofIgAexpression.Conclusion:FourcommonspeciesofSeaCucumberscansignificantlyprotectthesmallintestinemucosaofmicefromtheCTXinduceddamage,andtheprotectiveeffectsweremoreobviousinthemiceofApostichopusjaponicusandThelenotaananasgroups.Keywords:seacucumber;cyclophosphamide;intestinalmucosalimmunity;Th1/Th2;V/C;SIgA肠道不仅是机体的消化吸收器官,同时还是机体最大的“天然免疫器官”。肠道粘膜本身的结构和功能,及肠道内部的有益菌,可构成强...