雷帕霉素纳米粒局部处理兔离体静脉对防治其移植后狭窄的作用VIP免费

雷帕霉素纳米粒局部处理兔离体静脉对防治其移植后狭窄的作用巩性军曹广庆张希全吴树明张供李跃华周敏[摘要]目的研究用纳米缓释技术处理的雷帕霉素(RPM-NP)对移植静脉狭窄的防治作用。方法选取家兔40只，建立兔颈外静脉间位移植至颈总动脉的移植静脉模型，按处理方式不同，分为4组：药物纳米局部干预组（RPM-NP局部处理颈外静脉，n=10，A组）、药物纳米全身干预组(RPMNP周围静脉滴注，n=10，B组)、空白纳米局部干预组（空白纳米粒局部处理颈外静脉，n=10，C组）和未干预组（不做上述处理，n=10，D组）。术后28d，切取各组移植静脉和对侧正常静脉，病理学检测移植静脉内膜厚度、内径、内膜/中膜厚度比以及胶原容积指数。结果移植静脉的组织病理学检测结果表明，A、B、C、D组移植静脉和对侧正常静脉的内膜/中膜厚度比分别为0.26±0.02、0.73±0.05、0.71±0.04、0.69±0.03、0.24±0.01，胶原容积指数比分别为0.24±0.03、0.56±0.06、0.53±0.07、0.49±0.08、0.21±0.01。B、C、D组的组织病理学（移植静脉内膜厚度、内径、内膜/中膜厚度比以及胶原容积指数）与对侧正常静脉组比较，均出现明显的病理学改变，差异均有统计学意义(均P＜0.05)；3组之间移植静脉的组织病理学结果比较，差异均无统计学意义（均P＞0.05）。而A组移植静脉的组织病理学结果与对侧正常静脉比较，差异无统计学意义(P＞0.05)；与其他3组比较，差异有统计学意义(P＜0.05)。结论采用纳米缓释技术处理的雷帕霉素，对离体的移植静脉行管腔内局部处理,能抑制移植静脉内膜增生,防治移植静脉狭窄。西罗莫司；纳米粒子；局部治疗；移植静脉；狭窄PretreatmentofisolatedveinwithrapamycinnanopartidesinhibitsveingraftstenosisinrabbitsGONGXing-junCAOGuang-qingZHANGXi-quanWUShu-mingZHANGGongLIYue-huaZHOUDepartmentofCardiovascularSurgery,QiluHospitalofShandongUniversity,Jinan250012,China[Abstract]ObjectiveToexploretheeffectsofpretreatmentofcarbopol-encapsulatedrapamycinloadednanoparticles(RPM-NP)onveingraftstenosisinarabbitveingraftmodel.MethodsAsegmentofcommoncarotidarterywasreplacedwithasegmentofexternaljugularveinin40rabbits.Theywereseparatedintofourtreatmentgroups,i.e.GroupA:veingraftswerepretreatedwithintraluminalRPM-NPperfusion;GroupB:peripheralvenousveinswereinjectedwithRPM-NP;GroupC:veingraftsreceivedanequivalentperfusionofemptyvehicle;GroupD:veingraftsreceivednotreatment.AtDay28postoperation,thegraftsandnormalveinswereharvestedforhistologicalexaminationstoanalyzetheindicatorsofintimalthickness,internaldiameter,intimal/mediathicknessratioandcollagenvolumeindex.ResultsAtDay28post-operation,theintimal/mediathicknessratioswere0.26±0.02,0.73±0.05,0.71±0.04,0.69±0.03and0.24±0.01inGroupsA,B,CandDandthenormalvein;thecollagenvolumeindex0.24±0.03,0.56±0.06,0.53±0.07,0.49±0.08and0.21±0.01respectively.Comparedwiththenormalveins,thepathologicalindicatorsofveingraftintimalthickness,internaldiameter,intimal/mediathicknessratioandcollagenvolumeindexhadsignificantdifferencesinGroupsB,CandD(allP＜0.05).Buttherewerenosignificantdifferencesamong3groups(allP＞0.05).Comparedwiththenormalvein,theparametersofveingraftintimalthickness,internaldiameter,intimal/mediathicknessratioandcollagenvolumeindexhadnosignificantdifferenceinGroupA(allP＞0.05).Butascomparedwithothergroups,theseindicatorshadstatisticalsignificantdifferenceinGroupA(allP＜0.05).ConclusionThelocalpretreatmentofisolatedveinwithrapamycinnanoparticlesmayinhibitneointimalhyperplasiaandpreventeffectivelyveingraftstenosis.Sirolimus;Nanoparticles;Localtreatment;Veingraft;Stenosis10.3760/cma.j.issn.0376-2491.2011.46.017山东省卫生厅医药卫生发展科技项目(2007HZ057)250012济南，山东大学齐鲁...