血清HMGB1、esRAGE水平与2型糖尿病颈动脉粥样硬化的相关性胡晓姣,荆爱玉,谢璇,吴娟娟,张静,滕云(西安交通大学医学院第二附属医院内分泌科,陕西西安710004)摘要:目的探讨血清HMGB1、esRAGE与2型糖尿病颈动脉粥样硬化的关系。方法选取198例住院患者作为病例组,根据是否存在2型糖尿病及颈动脉彩超检测结果,将病例组分为3组,分别为单纯颈动脉粥样硬化组(AS)72例;单纯2型糖尿病组(T2DM)56例;2型糖尿病合并颈动脉粥样硬化组(T2DM+AS)70例。同时选取46例健康体检者作为正常对照组。应用酶联免疫吸附试验(ELISA)测定血清HMGB1、esRAGE。结果T2DM+AS组患者血清HMGB1较其他组明显升高(P<0.05),esRAGE显著降低(P<0.01);T2DM+AS组血清HMGB1与esRAGE呈负相关(r=-0.317,P=0.002);Logistic回归分析结果显示,HMGB1、esRAGE与2型糖尿病合并颈动脉粥样硬化独立相关(OR=1.202,P=0.021,95%CI1.028~1.406;OR=0.301,P=0.046,95%CI0.004~0.848)。结论外周循环中高水平的HMGB1及低水平的esRAGE可能参与2型糖尿病合并颈动脉粥样硬化的发生发展。关键词:2型糖尿病;高迁移率蛋白B1;内源性分泌型糖基化终末产物受体;动脉粥样硬化中图分类号:R587.1文献标志码:A文章编号:1671-8259AssociationofHMGB1andesRAGElevelswithcarotidatherosclerosisintype2diabetesmellitusHUXiao-jiao,JINGAi-yu,XIEXuan,WUJuan-juan,ZHANGJing,TENGYun(DepartmentofEndocrinology,theSecondAffiliatedHospital,MedicalSchoolofXi’anJiaotongUniversity,Xi’an710004,China)ABSTRACT:ObjectiveToexploretherelationshipofhighmobilitygroupbox-1protein(HMGB1)andendogenoussecretoryreceptorforadvancedglycationendproducts(esRAGE)withcarotidatherosclerosis(AS)intype2diabetesmellitus(T2DM).MethodsWerecruited198hospitalizedpatientsasthepatientgroup.BasedonthepresenceofT2DMandcarotidultrasonographytestresults,thepatientgroupwassubdividedintoAS(72cases),T2DM(56cases)andT2DM+AS(70cases)groups;46healthysubjectswereselectedasthenormalcontrolgroup.SerumlevelsofHMGB1and收稿日期:2012-12-06修回日期:2013-04-16基金项目:陕西省科技攻关项目(2012K16-06)SupportedbytheScientificandTechnologicalProjectsofShaanxiProvince(2012K16-06)通讯作者:荆爱玉,副教授,硕士研究生导师.E-mail:jingay@163.com作者简介:胡晓姣(1986-),女(汉族),在读硕士研究生.研究方向:糖尿病及慢性并发症研究.E-mail:huxiaojiao1987@163.comDOI:CNKI:61-1399/R.20130708.1123.002网络出版时间:2013-07-0811:23网络出版地址:http://www.cnki.net/kcms/detail/61.1399.R.20130708.1123.002.htmlesRAGEweredeterminedbyenzyme-linkedimmunosorbentassay(ELISA).ResultsHMGB1levelwassignificantlyhigher(P<0.05)andesRAGElevelwaslower(P<0.01)inT2DM+ASgroupthanintheother3groups.TherewasanegativecorrelationbetweenHMGB1andesRAGEinT2DMwithcarotidAS(r=-0.317,P=0.002).LogisticregressionanalysisindicatedthatHMGB1andesRAGElevelswereindependentlyassociatedwithcarotidASinT2DM(OR=1.202,P=0.021,95%CI1.028-1.406;OR=0.301,P=0.046,95%CI0.004-0.848).ConclusionIncreasedHMGB1anddecreasedesRAGElevelintheperipheralcirculationmayparticipateinthepathogenesisandprogressionofASinT2DM.KEYWORDS:type2diabetesmellitus;highmobilitygroupbox-1protein(HMGB1);endogenoussecretoryreceptorforadvancedglycationendproduct(esRAGE);atherosclerosis高迁移率蛋白B1(high-mobilitygroupbox1,HMGB1)是近年发现的一种晚期炎症介质[1]。研究显示HMGB1在动脉粥样硬化(atherosclerosis,AS)组织的单核-巨噬细胞、内皮细胞和血管平滑肌细胞中高表达,而且随着动脉硬化程度的增加,表达HMGB1的巨噬细胞数量也随之增加。糖尿病患者长期高血糖引起蛋白质的非酶糖基化,导致糖基化终末产物(advancedglycationendproducts,AGEs)和HMGB1的形成和堆积,AGEs则通过与糖...