纤维素酶产生菌的分离及其酶活力测定VIP专享VIP免费

安徽农业科学。JournalofAnhuiA卵．Sci．2009．37C35)：17323—17325．17357责任编辑王森责任校对卢瑶纤维素酶产生菌的分离及其酶活力测定巫小琴1,2徐强1，李羰1，陶诗1，黎勇h(1．内江师范学院．四川省高校特色农业资源研究与利用重点实验室，四川内江641112；2．四川省德阳市绵竹市玉泉学校，四川绵竹618219)摘要[目的]从自然界中分离纯化纤维素酶高产菌株并测定各茵株的最适发酵时问。【方法]利用刚果红变色圈法从花台土、湿地、菜地土、草坪土中分离产纤维素酶活力较高的茵样，通过茵落形态、茵体形态镜栓及革兰氏染色反应鉴定为3个不同种类的4株放线茵，分剐命名为Al、A2、A3。用DNS分光光度法对其所产纤维素酶活力分剐进行神l定，并与枯草芽孢杆茵(Bacillussubtilis)、青霉(Penicilli-啪)所产的纤维素酶活力进行比较。[结果]菜地土中和草坪土中产纤维素酶茵酶活力分别比枯草芽孢杆菌酶活力高出18．14％、40．50％，而比青霉酶活力高出39．96％、66．45％；花台土中和湿地中的纤维素酶产生茵酶活力则分别比枯草芽袍杆茵酶活力低12．97％、13．18％，而比青霉酶活力高出3．11％、2．86％。此外，各菌株所产纤维素酶活力均与发酵时间密切相关，表现出明显的阶段性：初始阶段纤维素酶活力呈上升的趋势，各菌株分别在不同发酵时间达到酶活力最大值．之后开始逐渐下降，回归于初始水平．[结论]在从自然界中分离纤维素酶高产菌株的时候一定要把握好取样地点；培养时间的不同对不同茵株产酶的影响很大；选育纤维素酶高产茵株可以提高纤维素酶的工业生产效率。关键词纤维素酶产生茵：分离；酶活力；分析中图分类号s182文献标识码A文章编号0517—6611(2009)35—17323—03IsolationofCellilla辨．producingBacteriaandDetermination仰ThdrEnzymeActivitiesWUXiao-qinetal(NeijiangNormalUniversity，KeyLaI，【)ratoryforResearchandUtilizationonRegionalSpecialA鲥cult吼lResourcesof"CollegesandUniversitiesinSiehuanProvince，Neijiang，Sichuan“l112)Abstract『0biective1Theaimwastoisolateandpurifyefficienteellulase．producingbacteriafromnatureanddeterminetheiroptimumfe卜mentationtime．『Method]Thestrainsproducinghisheradivecellulasewerei∞latedfromflower．standsotl，moistsolI，vegetablefieldsetlandlawnsotlbyCongodi∞oloratloncirclemethodandtheywereidentifiedas4strainsofActinomvcesof3differentspeciesbymicroscopicalexaminationontheirCO]OHVshapesandsomaticshapesandGram’SstainingandnamedA1．A2andA3．111eircell,,laseactivitiesweredeter-minedbyDNSSlDectrophotometryandcomparedwiththatofBacill船subtilisandPenicillium．[Result]Itwasshownintheresultthattheeel·lula．$eactivitiesofeellulase．producingbacteriafromvegetablefieldsotlandlawnsotlwereenhancedby18．14％and40．50％resp．incorn-parisonwiththatofB．subtilisandenhancedby39．96％and66．45％resp．incomparisonwiththatofPenicillium：thecellulaseactivitiesofcellulase．producingbacteriafromflower．stand∞i】andmoistsotlweredecreasedbyabout12，97％and13．18％resp．incomparisonwiththatofB．subtilisandincreasedby3．11％and2．86％resp．incomparisonwiththatofPenic豇lium．Besides．theactivitiesofceUulasesproducedbyvariousstrainshadcloserelationshipwithfermentationtime．showingobviousstage：thecellulaseactivityshowedincreasingtrendinpre·liminarystage．thecellulaseactivitiesofvariousstrainsreachedtheirmaximumsindifie．rentfermentationlime，andthentheircellulmseactivi-tiesbegantodecreasegraduallyandreturntotheirinitiallevel．[Conclusion]Samplingspotw鹤thekeytoisolatethecellulase·producingbacteriafromthenature．ThediⅡjrenceofculturetimehadgreatinfluenceonthecelhdasc-productionsofdiffe·rentstrains．Theindustrialp胁ductionefficiencyofcellulasecouldbeenhancedhbreedinge侬cientcellulase—p...