http://www.paper.edu.cn-1-AnalysisofSulfamerazineinPondWaterandSeveralFishesbyHigh-PerformanceLiquidChromatographyUsingaMolecularlyImprintedSolid-PhaseExtractionGuoLinyuan，JiangXiaoman，YangCailing，ZhangHaixia*DepartmentofChemistry，LanzhouUniversity，Lanzhou（730000）E-mail：zhanghx@lzu.edu.cnAbstractThesynthesisandevaluationofamolecularlyimprintedpolymer(MIP)asaselectivesolid-phaseextractionsorbent,coupledtohigh-performanceliquidchromatography(HPLC),fortheefficientdeterminationofsulfamerazine(SMR)inpondwaterandthreefishesarereported.ThepolymerwaspreparedusingSMRasthetemplatemolecule,methacrylicacidasthefunctionalmonomerandethyleneglycoldimethacrylateasthecross-linkingmonomerinthepresenceoftetrahydrofuranasthesolvent.TheSMR-imprintedpolymersandnon-imprintedpolymerswerecharacterizedbyFT-IRandthestaticadsorptionexperiments.ThepreparedSMR-imprintedmaterialshowedhighadsorptioncapacity,significantselectivityandgoodsiteaccessibility.ThemaximumstaticadsorptioncapacityoftheSMR-imprintedandnon-imprintedmaterialforSMRwas108.8and79.6mgg-1,respectively.TherelativelyselectivefactorvalueofthisSMR-imprintedmaterialwas1.6.Severalparametersinfluencingthesolid-phaseextractionprocesswereoptimized.Atlast,theSMR-imprintedpolymerswereusedasthesorbentinsolid-phaseextractiontodetermineSMRinpondwaterandthreefisheswithsatisfactoryrecovery.Thelimitofdetection(LOD)forSMRwas1ngg-1andthelimitofquantitation(LOQ)was3.5ngg-1.Keywords：Molecularlyimprintedpolymers(MIPs)；Solid-phaseextraction；Sulfamerazine(SMR)；High-PerformanceLiquidChromatography(HPLC)1．IntroductionMolecularlyimprintedpolymers(MIPs)areanartificiallysynthesizedmacromolecularmaterial,whichiseffectiveinencodingmolecularinformation(shape,sizeandfunctionalgroupsorientation)inbulkmaterials[1,2].Inthelastfewyears,MIPswerewidelyusedfortheselectiveenrichmentandpretreatmentoftargetcompoundsexistingincomplexmatrix.Andmolecularimprintingtechnologyhasbeenexpandedtothefieldofenvironmentalanalysis[3-7].Thesynthesisofmolecularlyimprintedpolymers(MIPs)involvestheformationofacomplexbetweenatemplatemoleculeandthefunctionalmonomersinanappropriatesolvent.Afterpolymerization,thetemplatemoleculeisremovedandleavingmicrocavitiesthatarecomplementarytothetemplatemolecule.Theadvantagespresentedbyimprintingtechniquesarenumerous,asthesepolymericmaterialsarerobusttoextremesoftemperatureandpH,andcanretaintheirrecognitionpropertiesoverperiodsofseveralyears[8].Theirreusabilityandshorterpreparationtimesarealsotheirobviousadvantages.GreateffortshavebeendirectedtoapplythemolecularrecognitioncapabilityofMIPstosolidphaseextraction[9,10].Solid-phaseextraction(SPE)hasbecomearoutinetoolinmanylaboratoriesforpre-concentrationand/orclean-upstepsintheanalysisofnumerousenvironmentalandbioanalyticalsamples[11-15].DuetothepopularityofSPE,newsorbentshaveappearedasalternativetoconventionalsorbentswiththeaimofachievingamoreandmoreselectivepreconcentrationoftargetanalytes.Thus,molecularlyimprintedpolymers(MIPs),reliedonaffinityinteractionsandpotentiallyofferahigherdegreeofsampleclean-upefficiencythanthatachievedusingconventionaltypeSPEsorbents[16],appearasexcellentcandidatestoaccomplishthisrequirement[17].TheapplicationofMIPtechnologytosolid-phaseextraction(MISPE)hasbeenusedforbothbiologicalandenvironmentalsamplesandhasbeenreviewedindetail[18-21].ThemajorityofstudiesintoMISPEhaveus...
