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应用分子印记固相萃取法结合高效液相色谱测定池塘水及三种鱼肉中磺胺甲基嘧啶含量VIP免费

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http://www.paper.edu.cn-1-AnalysisofSulfamerazineinPondWaterandSeveralFishesbyHigh-PerformanceLiquidChromatographyUsingaMolecularlyImprintedSolid-PhaseExtractionGuoLinyuan,JiangXiaoman,YangCailing,ZhangHaixia*DepartmentofChemistry,LanzhouUniversity,Lanzhou(730000)E-mail:zhanghx@lzu.edu.cnAbstractThesynthesisandevaluationofamolecularlyimprintedpolymer(MIP)asaselectivesolid-phaseextractionsorbent,coupledtohigh-performanceliquidchromatography(HPLC),fortheefficientdeterminationofsulfamerazine(SMR)inpondwaterandthreefishesarereported.ThepolymerwaspreparedusingSMRasthetemplatemolecule,methacrylicacidasthefunctionalmonomerandethyleneglycoldimethacrylateasthecross-linkingmonomerinthepresenceoftetrahydrofuranasthesolvent.TheSMR-imprintedpolymersandnon-imprintedpolymerswerecharacterizedbyFT-IRandthestaticadsorptionexperiments.ThepreparedSMR-imprintedmaterialshowedhighadsorptioncapacity,significantselectivityandgoodsiteaccessibility.ThemaximumstaticadsorptioncapacityoftheSMR-imprintedandnon-imprintedmaterialforSMRwas108.8and79.6mgg-1,respectively.TherelativelyselectivefactorvalueofthisSMR-imprintedmaterialwas1.6.Severalparametersinfluencingthesolid-phaseextractionprocesswereoptimized.Atlast,theSMR-imprintedpolymerswereusedasthesorbentinsolid-phaseextractiontodetermineSMRinpondwaterandthreefisheswithsatisfactoryrecovery.Thelimitofdetection(LOD)forSMRwas1ngg-1andthelimitofquantitation(LOQ)was3.5ngg-1.Keywords:Molecularlyimprintedpolymers(MIPs);Solid-phaseextraction;Sulfamerazine(SMR);High-PerformanceLiquidChromatography(HPLC)1.IntroductionMolecularlyimprintedpolymers(MIPs)areanartificiallysynthesizedmacromolecularmaterial,whichiseffectiveinencodingmolecularinformation(shape,sizeandfunctionalgroupsorientation)inbulkmaterials[1,2].Inthelastfewyears,MIPswerewidelyusedfortheselectiveenrichmentandpretreatmentoftargetcompoundsexistingincomplexmatrix.Andmolecularimprintingtechnologyhasbeenexpandedtothefieldofenvironmentalanalysis[3-7].Thesynthesisofmolecularlyimprintedpolymers(MIPs)involvestheformationofacomplexbetweenatemplatemoleculeandthefunctionalmonomersinanappropriatesolvent.Afterpolymerization,thetemplatemoleculeisremovedandleavingmicrocavitiesthatarecomplementarytothetemplatemolecule.Theadvantagespresentedbyimprintingtechniquesarenumerous,asthesepolymericmaterialsarerobusttoextremesoftemperatureandpH,andcanretaintheirrecognitionpropertiesoverperiodsofseveralyears[8].Theirreusabilityandshorterpreparationtimesarealsotheirobviousadvantages.GreateffortshavebeendirectedtoapplythemolecularrecognitioncapabilityofMIPstosolidphaseextraction[9,10].Solid-phaseextraction(SPE)hasbecomearoutinetoolinmanylaboratoriesforpre-concentrationand/orclean-upstepsintheanalysisofnumerousenvironmentalandbioanalyticalsamples[11-15].DuetothepopularityofSPE,newsorbentshaveappearedasalternativetoconventionalsorbentswiththeaimofachievingamoreandmoreselectivepreconcentrationoftargetanalytes.Thus,molecularlyimprintedpolymers(MIPs),reliedonaffinityinteractionsandpotentiallyofferahigherdegreeofsampleclean-upefficiencythanthatachievedusingconventionaltypeSPEsorbents[16],appearasexcellentcandidatestoaccomplishthisrequirement[17].TheapplicationofMIPtechnologytosolid-phaseextraction(MISPE)hasbeenusedforbothbiologicalandenvironmentalsamplesandhasbeenreviewedindetail[18-21].ThemajorityofstudiesintoMISPEhaveus...

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应用分子印记固相萃取法结合高效液相色谱测定池塘水及三种鱼肉中磺胺甲基嘧啶含量

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