ChineseJournalofReparativeandReconstructiveSurgery,October2012,Vol.26,No.10·1218·小鼠胫骨中段1/3不同缺损直径单层骨皮质缺损模型比较研究李福兵1徐永清1潘兴华2李霞1刘华1杨杜明1李军1沙勇1石健1赵万秋1【摘要】目的比较研究不同缺损直径对小鼠胫骨中段1/3单层骨皮质缺损模型愈合的影响，为组织工程材料研究、骨缺损修复及其分子机制研究和骨缺损基因治疗研究等提供动物模型。方法取8周龄雄性C57BL/6J小鼠10只，体重（20±2）g，随机分为A、B两组，每组5只。利用牙科磨钻分别制备直径为0.8mm（A组）和1.0mm（B组）小鼠胫骨中段1/3单层骨皮质缺损模型。于造模后7、21、28d摄钼靶X线片观察缺损修复情况；28d对骨缺损修复行MicroCT扫描及骨组织三维成像；28d取材行HE染色观察。结果B组5只小鼠造模7d内均发生二次骨折，A组无骨折发生。X线片、MicroCT和HE染色均显示A组胫骨单层骨皮质缺损可在28d达骨性愈合。MicroCT定量分析骨小梁示，A组骨小梁数目、骨小梁密度、骨体积显著高于B组，骨质密度显著低于B组，差异均有统计学意义（P<0.05）；两组骨小梁分离度、骨小梁厚度差异无统计学意义（P>0.05）。结论小鼠胫骨中段1/3单层直径0.8mm骨皮质缺损模型是研究胫骨缺损无外固定缺损修复机制和骨替代植入材料的理想动物模型。【关键词】胫骨骨缺损动物模型小鼠ACOMPARATIVESTUDYONEFFECTOFDIFFERENTDEFECTDIAMETERSONHEALINGINMIDDLE1/3TIBIAMONOLAYERCORTICALBONEDEFECTMOUSEMODEL/LIFubing1,XUYongqing1,PANXinghua2,LIXia1,LIUHua1,YANGDuming1,LIJun1,SHAYong1,SHIJian1,ZHAOWanqiu1.1DepartmentofOrthopaedics,ResearchInstituteofOrthopaedicTraumaofChinesePLA,2CenterforStemCellandOrganEngineering,KunmingGeneralHospitalofChengduMilitaryCommand,KunmingYunnan,650032,P.R.China.Correspondingauthor:XUYongqing,E-mail:xuyongqingkm@163.net【Abstract】ObjectiveTocomparetheeffectofdifferentdefectdiametersonhealinginthemiddle1/3tibiamonolayercorticalbonedefectmousemodelsoastoestablishananimalmodelforbonetissueengineeringstudy,mechanismstudyonbonedefectrepair,andgenetherapyresearch.MethodsTen8-week-oldC57BL/6Jmice,weighing(20±2)g,wererandomlydividedinto2groups,5miceineachgroup.Themiddle1/3tibiaemonolayercorticalbonedefectmodelof0.8mm(groupA)or1.0mm(groupB)indiameterwasestablishedwithburrdrill.At7,21,and28daysaftermodeling,themolybdenumtargetX-rayradiographywasusedtoobservethedefectrepair;at28days,MicroCTandthree-dimensionalimagingwereusedtoevaluatebonedefectrepair,andtibiaspecimenswereharvestedforHEstaining.ResultsAt7daysaftermodeling,tibiafractureoccurredin5miceingroupB,nofractureingroupA.X-rayfilms,MicroCTscan,andHEstainingshowedbonyunioningroupAat28days.ThequantitativeanalysisoftrabecularbonebyMicroCTshowedthattrabecularnumber,connectivitydensity,andbonevolumeingroupAweresignificantlygreaterthanthoseingroupB(P<0.05),meanofsegmentedregion—mean2wassignificantlylessthanthatingroupB(P<0.05),butnosignificantdifferencewasfoundintrabecularseparationandtrabecularthicknessbetween2groups(P>0.05).ConclusionThemiddle1/3tibiamonolayercorticalbonedefectmousemodelof0.8mmindiameteristheidealanimalmodelforstudyrepairmechanismoftibiadefectorbonetissueengineering.【Keywords】TibiaBonedefectAnimalmodelMouseFoundationitems:NationalNaturalScienceFoundationofChina(81171734);PostdoctoralScienceFoundationofChina(20100481529);AppliedBasicResearchProjectsofYunnanProvince(2011FZ312);HospitalSubjectofKunmingGeneralHospital(2009Y050C)基金项目：国家自然科学基金面上项目（81171734）；中国博士后科学基金面上项目（20100481529）；云南省应用基础研究项目（2011FZ312）；昆明总医院院管课题（2009Y050C）作者单位：成都军区昆明总医院（昆明...
