使用sequin序列提交GenebankVIP免费

准备工作：1.下载光quin软件https://www.ncbi.nlm.nih.gov/projects/Sequin/download/seq_win_download.html2.将需要递交的序列编辑成以下形式遴ITS.tidt-—□>窓饨F)無国旧囲冋吏W(V)餌取H)?-C161.organic」L=Diapcirthmfalclspora][straLn=C161_[iso1atiQn_£OUTCe=SaccharmnspontaneunjCDuntry=ChLna]strainCL6113SribosomalKNigene,partialsequence:internaltranscribedspacer1,5.8SrJbosomalRNAgene,andinternaltranscribedspacer2,conupLetesequencAand28Sr:bosoma1SNAgsns,partialsequenceTT^TTCOOCCrCCGT.'J^GGTG^CTGCGGAGGGMCATTMAMGOGAGTCTaGCGACTCTCAA^CCTATCTGAACTTMTCATGTCTGMCTGCT丁CGGC峽GCGTTKGMAG肚优MGTGGCGGCCGGCTEGCAACC血CTCTTTGTUTCTPLTGGMGTCTGMTT血脱AMATCTATTAM甌7nCMCMOGGArCTCTTGGTTCTGGCXTCGATGAAGAAOGCAGCGAAATGOGATAA&TAATGTGAAnGCAGAATTCAGTGAATCATOGAATCnTGAAOGC^AnGCGCCCTCTAGimCTGG^GGCATGCCTGnCGAGCGTCATnCA^COCTX^GrACCCTTGGGT^TAGGTGnGGGG^CCGCGGiMCGCGGCCCCCTWWACATTGGCGG^CTCCTMGGTATCCGAGC&CAGT^TMCMTCTCGCTCTGGGA^CCGG&MGGCnCCTGCCGnAMCCCCCAAniTTrAAGGTGACCTCGGATCAGGTAGAATGCOCX?197orga^.ism=Diaparthefalcispara]3traLn=C197l.isoLatian_soiiTCE=Difitaria1ibrosavar.yuHnanen=\s]country^ChinfilstrninC197L8SribosomalKMgene,partialsequence■nternatranscribedspacer1』5.SSribosomalRNAsens,andinternaltranscrIbedspacer2,completesequence,and2ESribosomalENAgene,par-.ialseQuencenn^TGATCCCTCCGTAWGTCWCCTGCGGAGGGATCATTAMMGCG^TCTOTC&^CTCTCWWCCCTATGT&^ACTTATTCATGTCTGAGCTGCTTC(^^(XGTnCGAAJi£}AJ^C2iCCrGCaGGO?GGCTOGCCAAa!iA^TCTnGn，ArCTArGGJiCCTCTGAGnAAAAM!AAAATCTATTWJiCTTTCMCMCGGArCTCTTGCTTCTGGCMCGATGMGMCGC^CGAAATGCGATAAGTAATGTGAAnGCAGMTTC^GTGMTCATCGAATCnTGMCGC^mGCGCCCTCTAGTAnCTGG^GGCATGCCTGnCGAGCGTCATnCA^CCCTCTGTACCCTTGGGTAT^GTGnGGGG^CCGCGGW\ACGCG^OTCCTAMGACATTGGCGGJiCCrCCrAAGGTATCCGAGCGCAGr^TAAC^TrcrCGCTCIGGGMCCOGGGATGGCnCCTGOCGnAAACOCCCAAHTTTrAAGGTGACCTCGGATCAGGTAGAATGCOCM屏尊辑的麟旳间201S；VU21:22开始提交1.打开炊件，点击StartNewSubmissionWdccmetoSequinMiSequinSeQuinipplicaticmVersion15.50Standard.Release[Feb22201?]]fa.tiCaitHEoorBiat匕dmDlagyInEcrnation4H£LLi.hraryNedicLMH-ktion«2LTIEtitui：G5D£J.th(30L)456-2476Ln£o=qLL=ruo=s,七口七.丄l«=ng-th7957ba•玉>=HJSaBieaos1SaBieaosID:7-S-41Length:1133basesOrgamsnMusoocLorspU—S—41Strain.U-S-HCount...