鸡的大小黄白卵泡分离及总RNA的提取目录中文摘要：.................................................................................2关键词：.....................................................................................2Abstract：..................................................................................2前言.............................................................................................21.材料和方法.............................................................................41.1实验动物及其组织.........................................................41.2主要仪器和试剂.............................................................41.2.1实验仪器................................................................41.2.2实验试剂................................................................41.2.3溶液配制................................................................51.3实验操作步骤.................................................................51.3.1取样........................................................................51.3.2大小黄白卵泡的提取............................................61.3.3总RNA的提取........................................................61.3.4总RNA完整性鉴定................................................61.3.5总RNA内基因组DNA的去除................................61.3.6反转录反应............................................................71.3.7PCR反应................................................................711.3.8电泳检测...............................................................71.3.9PCR产物纯化........................................................71.3.10连接转化.............................................................82.实验结论............................................................................82.1鸡卵泡颗粒细胞............................................................82.2总RNA的检测结果........................................................92.3PCR检测.......................................................................93.分析讨论...............................................................................104.参考文献...............................................................................12中文摘要：实验前,在大量查阅各项相关研究资料的前提下，明2确了CART与蛋鸡卵巢中不同大小黄白卵泡中的雌激素（Estrodiol,E2）和孕酮（P）分泌量的关系。本实验运用显微外科手术技术分离蛋鸡的大小黄卵泡、大小白卵泡，并分别进行总RNA的提取，通过CARTmRNA表达量的差异对CART蛋白进行定位，再通过体外注射dsRNA对CART的表达进行抑制，阻断CART对蛋鸡卵巢中卵泡生长发育及排卵的影响，从而提高蛋鸡产蛋量，延长产蛋周期，降低料蛋比，进而通过饲养试验，确定调控效果，并将该技术进行全面示范推广。关键词：CART；卵泡；总RNA；dsRNA；Abstract：Accordingtotheinformationofrelatedresearchbeforeexperiments,wefoundouttherelationshipsbetweentheamountofestrogen(E2)andprogesterone(P),whichthelayer’sfollicleindifferentsizesecreted,andCART.Duringthisexperiment,weusedthemicrosurgicaltechniquetoisolatedifferentfolliclesincludingRhubarbfollicle(LYF),Smallwhitefollicle(SWF),yellowfollicle(SYF)andthefollicle(LWF).Otherwise,wehavepickedupthetotalRNArespectivelyandlocatedtheCARTproteinthroughthedifferentamountofCARTexpression.Finally,wehave3suppressedtheinfluencesofCARTonthefollicles’growthandovulationinovarianbymeansofinjectingdsRNAartificially.Astoimproveth...
