液质联用分析多肽实验条件的探索 应用化学专业VIP免费

液质联用分析多肽实验条件的探索摘要高效液相色谱-质谱(HPLC-MS)联用技术是近几年来发展起来的一项新的分离分析技术。它将应用范围极广的分离方法——液相色谱法与灵敏、专属、能提供结构信息的质谱法结合起来，成为一种重要的现代分离分析技术。液质联用为生产和科研提供了许多有价值的数据，解决了许多在此之前难以解决的分析问题本文主要探索液质联用的条件，并运用LC-MS仪对多肽进行了产品性质认定和分析杂质性质，希望能建立一套完整的液质联用体系。关键词：高效液相色谱-质谱，联用的条件，产品性质认定，杂质分析AbstractHigh-performanceliquidchromatography-massspectrometry(HPLC-MS)inconjunctionwithtechnologyinrecentyearsdevelopedanewseparationanalysistechniques.Itaverywiderangeofapplicationsofseparationmethods-liquidchromatographywithsensitive,proprietary,canprovideinformationonmolecularweightandstructureofthecombinationofmassspectrometryhasbecomeanimportantseparationofmodernanalysistechniques.HPLC-MSprovidelotsofvaluabledataforproductionandscientificresearch,andsolvemanypreviouslydifficultproblems.ThispapermainlyexplorestheLC-MSconditions,andtheuseofLC-ESI-MSinstrumentandanalysisofimpuritiesonthepropertiesoftheproducttodeterminethenatureofthepolypeptide,hopetoestablishacompletesetofliquidchromatography-massspectrometrysystem.KeyWords：HPLC-MS,incombinationwiththecondition,natureoftheproductidentification,impurityanalysis目录摘要...................................................................................................................IAbstract.......................................................................................................................II第一章前言.......................................................................................III第二章实验部分.............................................................................62.1实验目的.......................................................................................62.2实验原理.......................................................................................62.3实验仪器与试剂..................................................................82.4实验步骤.......................................................................................82.4.1管道连接.............................................................................82.4.2流动性制备......................................................................82.4.3一般参数配置...............................................................82.4.4实验步骤.............................................................................9第三章结果与讨论..................................................................103.1第一个实验.............................................................................103.2第二个实验.............................................................................123.3第三个实验.............................................................................15第四章结论与展望..................................................................24参考文献..................................................................................................26致谢................................................................................................................27第一章前言蛋白质是生物体中含量最高、功能最重要的一类生物大分子。它存在于所有生物细胞中，约占细胞干质量的50%以上，它在生命科学中所占重要位置可想而知。LinusPauling[1]于1937年阐述...