紫外可见分光光度计基本原理及分析技术紫外可见分光光度计基本原理与分析技术紫外可见分光光度计基本原理与分析技术允许打印和进一步使用原文来自AnalytikJenaAG2007版翻译：汪素萍目录1定义和基本原理.......................................................................................................................51.1光的吸收及光谱................................................................................................................51.2透射和吸收........................................................................................................................61.3定性分析............................................................................................................................61.3.1光谱与结构.....................................................................................................................61.3.2影响光谱的因素...............................................................................................................71.3.3定性信息.........................................................................................................................71.4定量分析..............................................................................................................................81.4.1Lambert-Beer定律........................................................................................................81.4.2标准曲线的绘制.............................................................................................................81.4.3用于光度测量的样品制备.............................................................................................91.5动力学................................................................................................................................91.6UVVIS分光光度计的更多应用...................................................................................101.6.1蛋白和DNA分析...........................................................................................................101.6.2DNA熔点测定..............................................................................................................111.7导数光谱..........................................................................................................................121.7.1色度分析.......................................................................................................................131.7.2膜厚度的测定.................................................................................................................142UVVIS分光光度计的结构...............................................................................................162.1UVVIS分光光度计的组成...........................................................................................162.2光源..................................................................................................................................162.3分光系统..........................................................................................................................162.3.1单色器系统................................................................................................................162.3.2多色器系统...................................................................................................................172.4光谱带宽........................
