双向凝胶电泳技术原理与应用VIP免费

双向凝胶电泳技术原理与应用Principle and application of two-dimensional gel electrophoresis姓名： XX 班级：检验本科 1113 班学号： XXX 【摘要】人类基因组计划与美国塞莱拉遗传信息公司于2001年在美国《科学》杂志和英国《自然》杂志联合宣布，他们绘制出了准确、清晰、完整的人类基因组图谱，至此，人类基因组计划已基本完成，随着后基因组时代的到来，蛋白质组学得到了空前的发展， 蛋白质组研究旨在揭示基因表达的真正执行生命活动的全部蛋白质的表达规律和生物功能。包括蛋白质组、 蛋白质组学、 功能蛋白质组学和结构基因组学等新的概念的提出，蛋白质组学已成为当今生物领域中极其活跃的学科。其中双向电泳（two-dimensional electrophoresis，2.DE）是蛋白质组研究的三大关键核心技术之一【 abstract】Human gene group plans and United States sailaila genetic information company Yu 2001 in United States Science under magazine and United Kingdom natural under magazine joint announced, they draws out has accurate, and clear, and complete of human gene Group map, at this point, human gene group plans has basic completed, as Hou gene group era of comes, protein group learn get has unprecedented of development, protein group research aimed at reveals gene express of real implementation life activities of all protein of express law and biological function. Includes proteome, proteomics, structural proteomics and functional genomics of new concepts, such as proposed, proteomics has become extremely active in the field of biological sciences. Two-dimensional gel electrophoresis (two-dimensional electrophoresis,2.DE) is one of the three key core technologies of proteome research 【关键词】 双向电泳；蛋白质组学；后基因组时代【 key words 】two-dimensional electrophoresis，2.DE proteomics Post-genomics era 【前言 】由于蛋白质的等电点和分子量是两个彼此不相关的重要性质，而2.DE同时利用了蛋白质间的这两个性质上的差异分离蛋白质，因此2.DE 的分离能力非常强大，它甚至能将细胞...