电脑桌面
添加小米粒文库到电脑桌面
安装后可以在桌面快捷访问

ips培养技术手册

ips培养技术手册_第1页
1/11
ips培养技术手册_第2页
2/11
ips培养技术手册_第3页
3/11
Cell Stem CellProtocol ReviewGu idelines and Techniqu es for the Generationof Indu ced Plu ripotent Stem CellsNimet Maherali 1,2 and Konrad Hochedlinger1,*1Massachusetts General Hospital Center for Regenerative Medicine and Cancer Center, Harvard Stem Cell Institute,Department of Stem Cell and Regenerative Biology, 185 Cambridge Street, Boston, MA 02114, USA2Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA*Correspondence: khochedlinger@helix.mgh.harvard.eduDOI 10.1016/j.stem.2008.11.008Direct reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) provides an invaluableresource for regenerative medicine, enabling the generation of patient-specific cells of any lineage withoutthe use of embryonic material. A variety of methods exist for iPSC derivation, all reliant upon manipulationof a select group of transcription factors. We compare the currently reported protocols, identify essentialsteps common to these methods, and suggest minimal criteria for defining fully reprogrammed cells. In ad-dition, specific procedures aimed to optimize reproducible iPSC derivation are presented, with an emphasison standardization of certain parameters for accurate comparison between independent experiments.The generation of pluripotent cells from differentiated adult cellshas vast therapeutic implications, particularly in the context ofin vitro disease modeling, pharmaceutical screening, and cellularreplacement therapies. In addition, the ability to revert somaticcells to an embryonic state provides a unique tool to dissectthe molecular events that permit the conversion of one celltype to another.Previously devised strategies to induce pluripotency, such assomatic cell nuclear transfer (cl...

1、当您付费下载文档后,您只拥有了使用权限,并不意味着购买了版权,文档只能用于自身使用,不得用于其他商业用途(如 [转卖]进行直接盈利或[编辑后售卖]进行间接盈利)。
2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。
3、如文档内容存在违规,或者侵犯商业秘密、侵犯著作权等,请点击“违规举报”。

碎片内容

ips培养技术手册

确认删除?
VIP
微信客服
  • 扫码咨询
会员Q群
  • 会员专属群点击这里加入QQ群
客服邮箱
回到顶部