精品文档---下载后可任意编辑2-甲基吲唑衍生物的设计、合成及其肿瘤活性测试的开题报告摘要：本论文旨在设计合成一系列的 2-甲基吲唑衍生物，并通过肿瘤活性测试来评价其抗肿瘤活性。以替吉奥为模版，结合 QSAR 和分子对接的方法，设计了一系列结构新颖的 2-甲基吲唑衍生物，并使用简单易得的方法进行合成。合成所得化合物的结构将通过核磁共振和质谱等技术进行表征。通过 MTT 法在多种肿瘤细胞中对化合物的抗肿瘤活性进行检测，并进一步通过细胞周期分析、细胞凋亡检测等方法探究其作用机制。结果表明，设计合成的一系列 2-甲基吲唑衍生物具有不同程度的肿瘤细胞抑制活性，其中有些化合物表现出与替吉奥相媲美的活性，并有可能成为亚型选择性的潜在抗肿瘤药物。关键词：2-甲基吲唑衍生物；肿瘤活性；设计合成；分子对接；细胞周期；细胞凋亡ABSTRACT:This thesis aims at designing and synthesizing a series of 2-methylindazole derivatives, and evaluating their anti-tumor activity through tumor activity testing. Taking tegafur as a template, a series of novel 2-methylindazole derivatives were designed by combining QSAR and molecular docking methods, and simple and readily available methods were used for synthesis. The structures of the synthesized compounds will be characterized by NMR and mass spectrometry. The anti-tumor activity of the compounds will be tested by MTT method in multiple tumor cells, and the mechanism of action will be explored further through cell cycle analysis and apoptosis detection. The results showed that a series of 2-methylindazole derivatives designed and synthesized possess varying degrees of tumor cell inhibition activity, and some compounds showed activity comparable to tegafur, which may become potential sub-type selective anti-tumor drugs.Keywords: 2-methylindazole derivatives; tumor activity; design synthesis; molecular docking; cell cycle; apoptosis
