精品文档---下载后可任意编辑Pelo 条件性基因敲除胚胎干细胞株的建立的开题报告摘要：在人类和其他生物体中，很多生理和病理过程都与通过基因敲除产生的体内条件性基因行为变化有关。通过 CRISPR/Cas9 技术，我们建立了一种条件性基因敲除胚胎干细胞株的方法，该方法通过组合 CRISPR技术和注射某种化合物或激素，可以在体内对特定基因进行快速而有效的敲除，从而实现条件性基因行为的调控。我们选择了一些具有重要生理或病理意义的基因，例如转录因子和信号转导分子，用这种方法进行条件性敲除。经验证，敲除后的细胞株表现出与未敲除细胞株不同的行为，包括细胞增殖、分化和对不同外部刺激的反应等。此外，我们还观察到敲除基因的体内表现和纯化条件下的表现有所不同。本讨论的结果表明，通过 CRISPR/Cas9 技术建立的条件性基因敲除胚胎干细胞株可以为生理和病理过程的讨论提供新的方法和工具。关键词：基因敲除；胚胎干细胞；条件性基因行为；CRISPR/Cas9技术Abstract:Many physiological and pathological processes in humans and other organisms are related to conditional gene behavior changes produced by gene knockouts. Here, we developed a method to establish conditional gene knockout embryonic stem cell lines using CRISPR/Cas9 technology. By combining CRISPR technology and injecting a certain compound or hormone, specific genes can be efficiently knocked out, achieving conditional gene behavior regulation.We selected important genes with physiological or pathological significance, such as transcription factors and signal transduction molecules, for conditional knockout. We found that the behavior of knockout cells differed from that of wild-type cells, including cell proliferation and differentiation, as well as responses to different external stimuli. In addition, we observed differences between in vivo and in vitro expression of the knocked-out gene.精品文档---下载后可任意编辑Our results suggest that the establishment of conditional gene knockout embryonic stem cell lines using CRISPR/Cas9 provides a new tool for the study of physiological and pathological processes.Keywords: Gene knockout; embryonic stem cells; conditional gene behavior; CRISPR/Cas9 technology.
