精品文档---下载后可任意编辑UPLc-MS 快速测定血清 1，5-脱水葡萄糖醇方法的建立及应用的开题报告摘要：1，5-脱水葡萄糖醇（1,5-AG）是一种新的生化指标，可用于血糖控制评估和糖尿病早期筛查。本讨论旨在建立一种快速测定血清 1,5-AG的方法，并应用于临床检测。采纳 UPLC-MS 技术进行分析，优化了前处理、分离和检测参数，建立了一种快速、准确、重现性好的方法。经过验证，该方法具有良好的线性范围（0.01-100 μg/mL）、检出限（0.01 μg/mL）、相关系数（r>0.999）、准确度（97.30%-102.80%）和精密度（RSD<3.28%）。应用该方法分析了 60 例健康人群和 60 例糖尿病患者的血清 1,5-AG 浓度，发现糖尿病患者的 1,5-AG 浓度显著低于健康人群（P<0.05），并与血糖控制有关。关键词：1,5-脱水葡萄糖醇；UPLC-MS；新的生化指标；糖尿病早期筛查Abstract:1,5-anhydroglucitol (1,5-AG) is a new biochemical marker that can be used for glycemic control assessment and early screening for diabetes. The aim of this study was to establish a rapid method for measuring serum 1,5-AG and to apply it to clinical detection. UPLC-MS technology was used for analysis, and the pretreatment, separation, and detection parameters were optimized to establish a rapid, accurate, and reproducible method. After validation, the method had a good linear range (0.01-100 μg/mL), detection limit (0.01 μg/mL), correlation coefficient (r>0.999), accuracy (97.30%-102.80%), and precision (RSD<3.28%). The method was applied to analyze the serum 1,5-AG concentrations of 60 healthy individuals and 60 diabetes patients. It was found that the 1,5-AG concentration in diabetes patients was significantly lower than that in healthy individuals (P<0.05) and was related to glycemic control.Keywords: 1,5-anhydroglucitol; UPLC-MS; new biochemical marker; early screening for diabetes.
