精品文档---下载后可任意编辑两株植物内生链霉菌次生代谢产物及其抗菌活性讨论的开题报告摘要本讨论旨在探究两株植物及其内生链霉菌次生代谢产物的抗菌活性，以期为寻找新型天然抗菌剂提供参考。首先，利用形态学、生理生化等方法对两株植物及其内生链霉菌进行鉴定和筛选，分离次生代谢产物，进一步通过质谱技术进行分析和鉴定。同时，采纳光学显微镜和荧光染色技术观察链霉菌生长和代谢产物的分布情况。最后，利用体外抗菌试验和菌落计数法评价产物的抗菌活性。预期结果为得到两株植物及其内生链霉菌中的次生代谢产物，并对其抗菌活性进行评价，结果可用于指导开发新型天然抗菌剂。关键词：植物；链霉菌；次生代谢产物；质谱技术；体外抗菌试验AbstractThe aim of this study is to explore the antimicrobial activity of two plants and their endophytic Streptomyces secondary metabolites, in order to provide reference for the discovery of new natural antimicrobial agents. Firstly, the identification and screening of two plants and their endophytic Streptomyces were performed using morphological, physiological and biochemical methods. The secondary metabolites were isolated and further analyzed and identified by mass spectrometry. At the same time, the growth of Streptomyces and the distribution of metabolites were observed using optical microscopy and fluorescence staining techniques. Finally, the antimicrobial activity of the products was evaluated using in vitro antibacterial tests and colony counting methods.The expected results are to obtain the secondary metabolites of two plants and their endophytic Streptomyces, and to evaluate their antimicrobial activity, which can be used to guide the development of new natural antimicrobial agents.Keywords: plants; Streptomyces; secondary metabolites; mass spectrometry; in vitro antibacterial tests
