微波辅助提取刺五加皮中的总黄酮

微波辅助提取刺五加皮中的总黄酮 摘 要本文对微波辐射提取刺五加皮中的黄酮类化合物进行了研究。利用紫外-可见分光光度标准曲线法测定所提取黄酮类化合物的含量，研究了提取剂乙醇浓度、料液比、微波提取时间、微波功率等因素对黄酮类化合物提取的影响，利用单因素实验和正交实验确定了最佳提取条件，即刺五加皮 粉 0.2000g，粉碎度60~80 目，溶剂乙醇浓度 50%，料液比 1：60（g/mL），提取时间 2min，微波功率为280W。此时，黄酮类化合物提取含量可达 16.51mg/g，实验的标准偏差 0.23mg/g，相对标准偏差为 1.4% ，加标回收率在 97.98%~101.57%之间的平均值为 99.77%。实验结果显示，微波辅助提取总黄酮类化合物具有操作简单方便、提取快速且提取率高、无污染、节约能源、时间短、溶剂用量少等优点，可为刺五加皮的深入开发利用提供参考依据，具有一定的应用价值。关键词：微波；刺五加皮；提取；黄酮类化合物IAbstractIn this paper, the extraction of flavonoids from Acanthopanax senticosus by microwave irradiation was studied.The content of flavonoids extracted by UV-visible spectrophotometry was determined by UV-visible spectrophotometry. The effects of ethanol concentration, solid-liquid ratio, microwave extraction time and microwave power on the extraction of flavonoids were studied. Orthogonal experiment determined the optimal extraction conditions, namely 0.2000g of Acanthopanax senticosus powder, 60~80 mesh pulverization degree, 50% solvent ethanol concentration, 1:60 (g/mL) ratio of material to liquid, extraction time 2 minutes, microwave The power is 280W. At this time, the extraction rate of flavonoids can reach 16.51, the standard deviation of the experiment is 0.23, the relative standard deviation is 1.4%, and the average of the spiked recoveries between 99.30% and 101.57% is 99.75%.The experimental results show that the extraction of total flavonoids by microwave irradiation has the advantages of simple and convenient operation, rapid extraction, high extraction...