Ⅰ同仁堂富硒麦芽蛋白粉中乳清蛋白代谢产物的组分分析目 录摘要 ............................................................ Ⅰ -Ⅱ1 前言 .............................................................. 1 2 材料与仪器 ........................................................ 2 2.1 仪器 ............................................................ 2 2.2 试剂............................................................23 方法..............................................................33.1 样品乳清蛋白的发酵代谢..........................................33.2 溶液的配制......................................................33.3 Sephadex 层析柱法（2.5×50cm）...................................33.4 蛋白质含量测定..................................................43.5 分离组分中总糖的测定............................................53.6 游离氨基酸总量的测定............................................63.7 多肽含量的测定..................................................63.8 内源荧光光谱扫描................................................73.9 褐变程度的测定..................................................73.10 ABTS 法测定抗氧化活性...........................................73.11 ORAC 法.........................................................74 结果..............................................................84.1 样品的鉴定与分离................................................84.2 分离得到各个组分具体数据及回收率.................................94.3 SDS-PAGE 凝胶电泳................................................94.4 分离组分中总糖、氨基酸、多肽及蛋白质的含量......................104.5 褐变程度.......................................................134.6 内源荧光光谱扫描...............................................134.7 考察分离组分的 ABTS+自由基清除率.................................144.8 比较各分离组分 ABTS+自由基清除的能力.............................154.9 比较各分离组分 ORAC 抗氧化活性相对值.............................155 讨论....
