1摘 要在生产完毕的茶树菇废料中仍含有大量的有机物质和各种营养成分，为探索滑菇菌种在液体培养基的不同茶树菇废料比例条件下对滑菇产生漆酶活性的影响，利用 ABTS（2，2-联氮-3-乙基-苯并噻唑-6-磺酸）的方法探讨茶树菇培养基废料比例对滑菇漆酶活性的影响。通过替代液体培养基的碳源和氮源分别设定为占总值的 0%、20%、40%、60%、80%、100%。通过单因素实验方法，主要是测定在相同培养基配方的不同废料比例的条件下滑菇菌丝每日的漆酶活性。通过单一变量实验研查废料比例对滑菇漆酶活性的影响，可为以后废料利用研究提供参考。关键词：滑菇；培养基；微碱化；ABTS 法；漆酶2AbstractThe waste of edible mushroom production still contains a variety of nutrients and a large number of organic substances. In order to explore the influence of shiitake mushroom on the laccase activity of shiitake mushroom in different proportion of waste of chashugu mushroom in liquid culture medium, the influence of the proportion of waste of chashugu mushroom culture medium on the laccase activity of shiitake mushroom was studied by using ABTS (2,2-diazo-3-ethyl-benzothiazole-6-sulfonic acid). The carbon source were set as 0%, 20%, 40%, 60%, 80% and 100% of the total value respectively. By single factor experiment, the laccase activity of shiitake mycelium was determined under the condition of different waste ratio of the same medium. The effect of waste proportion on laccase activity of shiitake mushroom was studied by single variable experiment, which can provide reference for the future study of waste utilization.Keywords: Shiitake; culture medium; micro alkalization; ABTS method; laccase3目 录1.引言............................................................11.2 滑菇漆酶测定..................................................11.3 漆酶..........................................................12. 材料与方法.....................................................32.2.1 原料........
